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Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl).

This method requires the following reagents

1. Orcinol reagent : 6% orcinol in 95% ethanol.

2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.))

Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test.

This method requires the following reagents

one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization.

Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution.

This method requires the following reagents

Hydrochloric acid, 1Normal

Schiff's reagent (made from pararosanilin treated with sulphurous acid)

Light green, 1% aqueous (can be replaced with Fast green FCF)

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12y ago

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