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An Autoclave is a piece of equipment used for sterilization purposes and requires quarterly calibration. There are three types of calibrations to be done - timer, temperature and pressure. To calibrate the timer, post an appropriate correction factor directly on the autoclave. Post a correction factor on the autoclave control panel if the temperatures were different, and for pressure post a correction factor on the autoclave if it is not true.

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Why you have to autoclave the test test tubes?

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What is the biological indicator to check the efficiency of autoclave?

Hi We can check the autoclave efficiency biologically by culturing MOs(eg.E.coli) on petridish and put it for 15-20min in the autoclave. Aftet that , we take the petridish and put it in the incubator,we can see it after 24h , if there is no growth of culture that means the autoclave is efficient. to make more sure, we take another plate & made a culture from the same source , then we incubate it directly .. if there is a growth of bacteria that mean this bacteria ia alive & the autoclave in the first time make good sterilization & killed them.


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