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We can check the autoclave efficiency biologically by culturing MOs(eg.E.coli) on petridish and put it for 15-20min in the autoclave.

Aftet that , we take the petridish and put it in the incubator,we can see it after 24h , if there is no growth of culture that means the autoclave is efficient.

to make more sure, we take another plate & made a culture from the same source , then we incubate it directly .. if there is a growth of bacteria that mean this bacteria ia alive & the autoclave in the first time make good sterilization & killed them.

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12y ago

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