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Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
For long-term storage, it is recommended to use a specialized cryoprotectant like glycerol and freeze the bacterial isolate at temperatures below -70°C in a dedicated freezer, such as a ultra-low temperature freezer. This method helps prevent ice crystal formation and maintain cell viability during storage, which is necessary to preserve the isolate effectively. Simply growing the bacteria in a broth with glycerol and storing in a normal freezer may not provide the necessary conditions for long-term preservation.
Where to find non pathogenic freeze dried staph bacteria with growth medium?
You can find non-pathogenic freeze-dried Staphylococcus bacteria with growth medium from scientific supply companies or online biological supply stores. These companies offer a variety of non-pathogenic bacterial strains for research and educational purposes. Make sure to follow appropriate safety protocols when working with bacteria in a lab setting.
What hapens to flowers after they freeze over night will they thaw out?
When flowers freeze overnight, the water inside their cells expands, causing the cell walls to rupture. This can lead to wilting and damage to the plant, and in severe cases, the flowers may not recover once they thaw out. It's best to try to protect flowers from freezing temperatures to prevent damage.
Does repeated freeze and thaw cause damage?
Yes, repeated freeze-thaw cycles can cause physical damage to materials such as concrete, rocks, and biological samples. This is due to the expansion and contraction of water as it freezes and thaws, leading to cracking and degradation of the material. It is important to minimize repeated freeze-thaw cycles in order to preserve the integrity of the material.
Do you water plants after a freeze?
You can water plants after a freeze, but it is much more important to make sure they are well-watered before the freeze. Most tissue damage occurs because of a lack of water inside the tissues, which then lose the heat energy from inside the cells (and the cells rupture when they freeze).
Do roses need to be covered in a freeze to protect them from damage?
Yes, roses should be covered in a freeze to protect them from damage, as the cold temperatures can harm the delicate petals and foliage of the plant.
Do you have to freeze a metal freeze plug?
No, they are just called that since they protect from damage if block freezes.
Where are freeze plugs located?
A freeze plug is located in the side of an engine block that is supposed to protect the block against freeze damage.
Should you water plants before a freeze to protect them from potential damage?
Yes, watering plants before a freeze can help protect them from potential damage by insulating the roots and providing some protection against the cold.
How important is it to consider watering plants before a freeze to protect them from potential damage?
It is very important to water plants before a freeze to help protect them from potential damage. Water acts as insulation and can help prevent the plant from freezing during cold temperatures.
How do you stabilize bacteria in liquid form?
It depends what you mean by stabilize. For long term storage of live bacteria, you can add glycerol to the liquid so that the final concentration of glycerol is 15% (I add 1ml of 60% glycerol to 3ml liquid). You can then freeze this without too much of the bacteria being destroyed by ice crystals. It is best to store them at -80ºC if you can, as they will be stable for longer than in a -20ºC freezer. If you just want the bacteria not to grow, then you can transfer them into phosphate buffered saline (PBS) and keep them in the fridge for a couple of days. If you want to 'fix' them, i.e. kill them but maintain their structure long-term, you can transfer them to 4% paraformaldehyde (i.e. 4g paraformaldehyde in 100ml PBS), or 95% ethanol.
Will potatoes survive a freeze, and if so, what measures can be taken to protect them from damage?
Yes, potatoes can survive a freeze, but they may be damaged if exposed to extremely low temperatures for an extended period. To protect potatoes from freeze damage, they should be harvested before the first frost, stored in a cool, dark place, and covered with a thick layer of mulch or straw to insulate them from the cold.
How can I protect my water plants before a freeze?
To protect water plants before a freeze, you can cover them with a blanket or tarp, bring them indoors if possible, or use a floating row cover. These methods can help insulate the plants and prevent damage from freezing temperatures.
Does watering plants before a freeze help protect them from the cold temperatures?
Watering plants before a freeze can help protect them from cold temperatures. The moisture in the soil can act as insulation and help regulate the plant's temperature, reducing the risk of frost damage.
Will aloe vera plants grow back after a freeze?
Yes, aloe vera plants can grow back after a freeze if the roots are not damaged. It is important to protect the plant from freezing temperatures to prevent damage.
When do plants freeze and how can we protect them from frost damage?
Plants freeze when temperatures drop below freezing, typically around 32 degrees Fahrenheit. To protect plants from frost damage, you can cover them with blankets or sheets, use frost cloths or row covers, water the plants before a frost to help insulate them, or bring potted plants indoors.
How important is it to water plants before a freeze to protect them from potential damage?
Watering plants before a freeze is important as it helps insulate the roots and soil, providing some protection from the cold temperatures. The water in the soil releases heat as it freezes, which can help keep the plant roots warmer. This can reduce the risk of damage to the plant during a freeze.
