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Transcription ends once the a termination sequence is reached. The sequence depends on which polymerase is being used and if the organism is a eukaryote or prokaryote.

In Eukaryotes when RNA polymerase 1 is used the termination sequence is an 18 nucleotide long sequence.

For RNA polymerase 3 the termination sequence is a short sequence of Uricils but the hairpin loop is not formed as it is in prokaryotes.

For RNA polymerase 2 transcription is terminated and cleavage takes place 10-35 nucleotides downstream of the AAUAA sequence.

For prokaryotes, termination can occur 2 ways. Termination can occur once the termination sequence is reached or using a protein called Rho factor.

For termination without Rho factor, the termination sequence is short and rich in Guanines and Cytosines followed by many Uricils in a row. A-U bonds are weaker than G-C bonds, the string of U-A bonds are easily broken and release the RNA strand

Using a Rho factor, once the Terminal sequence is reached, the Rho factor binds to a sequence 50-90 bases long and unwinds the DNA from the RNA , moving towards the 3' end, releasing the RNA

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