20 paltelets
The total magnification would be 750X (100X objective multiplied by 7.5X eyepiece). Oil immersion objectives are specifically designed for use with immersion oil to minimize light refraction and increase resolution when viewing specimens with high magnification.
The total magnification achieved when using a 100x oil immersion lens with a 10x binocular eyepiece is 1000x (100x objective magnification x 10x eyepiece magnification).
The oil immersion lens or objective has power 90X-100X and an eyepiece lens generally in light microscope comes with 10X so total magnification of oil immersion lens is 100X10 = 1,000
1. Focus very carefully with the 40x objective. 2. Rotate turret half way. 3. Apply a small drop of oil. 4. Rotate 100x objective into the immersion.
100x the higher the magnification the shorter the working distance
The field of view of the oil immersion 100x lens would be 0.4 mm. This is because the field of view decreases as the magnification increases.
100x with oil immersion
The oil immersion lens @100x
The total magnification would be 750X (100X objective multiplied by 7.5X eyepiece). Oil immersion objectives are specifically designed for use with immersion oil to minimize light refraction and increase resolution when viewing specimens with high magnification.
The total magnification achieved when using a 100x oil immersion lens with a 10x binocular eyepiece is 1000x (100x objective magnification x 10x eyepiece magnification).
A 100x oil immersion objective is best for studying bacteria under a microscope as it provides the highest magnification and resolution needed to observe bacterial morphology and structure in detail. Additionally, the use of oil immersion helps to reduce the refraction of light and improve image clarity.
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
The maximum magnification of a dark-field microscope typically ranges from 400x to 1000x. This level of magnification allows for detailed observation of specimens with enhanced contrast due to the dark-field illumination technique.
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
The oil immersion lens or objective has power 90X-100X and an eyepiece lens generally in light microscope comes with 10X so total magnification of oil immersion lens is 100X10 = 1,000
The objective lens referred to as the "high dry lens" is typically the 40x or 100x lens in a microscope, which allows for high magnification of specimens without the use of immersion oil. The term "dry" indicates that it operates without immersion oil, unlike the oil immersion lens, which is usually 100x. High dry lenses are useful for observing thin sections and mounted specimens, providing clarity and detail at higher magnifications.
The total magnification of a low power objective (LPO) is typically around 100x, a high power objective (HPO) is around 400x, and an oil immersion objective (OIO) can be up to 1000x. This means an oil immersion objective provides the highest magnification among the three.