The field of view of the oil immersion 100x lens would be 0.4 mm. This is because the field of view decreases as the magnification increases.
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
The total magnification of a microscope is calculated by multiplying the power of the objective lens by the power of the eyepiece lens. Given a total magnification of 200x and an eyepiece lens power of 10x, the power of the objective lens would be 200x/10x = 20x.
You would turn the nosepiece or turret on the microscope to switch from low power objective lens to a medium power objective lens. This allows you to change the magnification level and focus on different parts of the specimen being viewed.
In dark field microscopy, the light that reaches the objective only comes from light rays that are scattered by the object being observed. These scattered rays are then captured by the objective lens, illuminating the object against a dark background. The dark field condenser blocks the direct light rays that would otherwise create a bright background, allowing only the scattered light to form the image.
To find the total magnifying power of a microscope, you multiply the magnification of the objective lens by the magnification of the eyepiece. For example, if the objective lens magnifies 10x and the eyepiece magnifies 20x, the total magnifying power would be 10x * 20x = 200x.
You use the 3 objective lenses of a compound microscope to switch powers. There's LOW, MEDIUM, and HIGH power. With LOW power, you can magnify what you're looking at. With HIGH power, you can see things that you can't see with a naked eye.
To see the smallest part of a specimen, you would use the highest power objective lens available on the microscope, typically the 100x oil immersion objective. This lens allows for greater magnification and resolution, enabling the observation of fine details at the cellular or subcellular level. Additionally, using immersion oil helps to reduce light refraction and improve image clarity at this high magnification.
Depth of field is best demonstrated with a slide containing overlapping threads. The depth of field that would increase is the low power objective.
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
The total magnification would be 750X (100X objective multiplied by 7.5X eyepiece). Oil immersion objectives are specifically designed for use with immersion oil to minimize light refraction and increase resolution when viewing specimens with high magnification.
Changing to the High Power Objective would result in a larger magnification of the letter but a smaller field of view, making the letter appear larger but limiting the area visible around it. Additionally, increasing magnification might reduce the brightness of the field of view due to the reduced light-gathering ability of the higher power lens.
100x objective on the microscope is the most commonly used in microbiology because if you got a higher objective you would just have higher magnification. But you would not be able so see the details.
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
low-power because there's more of a field of depth where you can see more rather then in the high power you can't see as much and only one object will be in focus while everything else would be blurry in the background (if there's more then one thing in the slide)
Medium power objective gives the medium (as oppose to large or small), field of vision and the greatest depth of field. When you move the lens' position (CLose or far from the slide) it would be the middle. Read the textbook it will be of more benefit; or look it up on the internet you are on anyways. Type [edu] with brackets for a page of more scholarly links (if their domain is .edu).
The total magnification of a microscope is calculated by multiplying the power of the objective lens by the power of the eyepiece lens. Given a total magnification of 200x and an eyepiece lens power of 10x, the power of the objective lens would be 200x/10x = 20x.
The total magnification is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece lens. In this case, the total magnification would be 46x (objective) x 5x (eyepiece) = 230x magnification of the specimen.