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What is the role of radioactive phosphorus in DNA technologies?
To make DNA probes
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
In the Hershey and Chase experiment that helped confirm that DNA not protein was the hereditary material the key finding was that?
the key finding was that DNA, labeled with radioactive phosphorus, was the material that entered the bacterial cells during viral infection and was ultimately responsible for the transmission of genetic information, rather than the proteins also present in the viral coat.
Why did Hershey and Chase grow viruses in cultures that contained both radioactive Sulfur. What might have happened if they had used only one radioactive substance.?
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
Is phosphorescence radioactive?
Phosphorescence is not radioactive. It is a type of luminescence where energy absorbed from a light source is re-emitted slowly over a period of time, after the light source is removed. This process is different from radioactivity, which involves the emission of particles or energy from the nucleus of an unstable atom.
What happens when T2 phages are grown in the presence of radioactive phosphorus?
When T2 phages are grown in the presence of radioactive phosphorus, the phage DNA will incorporate the radioactive phosphorus into its structure during replication. This allows for visualizing the localization of the phage DNA within the infected bacterial cell using autoradiography.
When radioactive phosphorus decay it emits a positron will the resulting nucleus be another isotope of phosphorus?
No, whenever an atom emits a positron its atomic number is decreases by one unit (because a proton is converted into a neutron and a positron) but atomic mass remains the same so phosphorus is converted into silicon atom with same atomic mass.
Is phosphorus explosive radioactive or noxious?
yes it is
Is phosphorus stable or unstable?
Phosphorus is stable in its common forms, such as white phosphorus and red phosphorus. However, there are unstable isotopes of phosphorus that are radioactive and undergo decay.
33P is a radioactive isotope of phosphorus. Can 33P be used to make a radioactive nucleotide?
Yes, 33P can be used to label nucleotides and create radioactive nucleotides. This is because 33P is a radioactive isotope that can be incorporated into molecules, such as nucleotides, allowing researchers to track their behavior in biological systems.
Which molecule for which phosphorus-32 is used as a radioactive marker?
Dna
Does interstitial radiation use phosphorus?
Yes, interstitial radiation therapy can use radioactive isotopes of phosphorus, such as phosphorus-32, as a treatment for certain cancers. These radioactive sources are implanted directly into or near the tumor to deliver localized radiation therapy.
What is the role of radioactive phosphorus in DNA technologies?
To make DNA probes
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
Does phosphorus 32 occur naturally?
No, phosphorus-32 is a radioactive isotope of phosphorus and is not found naturally in significant amounts. It is typically produced in nuclear reactors or particle accelerators.
What is the difference between phosphorus 31p and 32p?
Phosphorus-31 (31P) is a stable isotope of phosphorus that is commonly used in nuclear magnetic resonance (NMR) spectroscopy, while phosphorus-32 (32P) is a radioactive isotope of phosphorus commonly used in biological research for labeling and tracking DNA and proteins. The main difference is the stability of the isotopes, with 31P being stable and 32P being radioactive.
How do you perform cold flame experiment with phosphorus?
To perform the cold flame experiment with phosphorus, you need white phosphorus, a glass container, a beaker of water, and safety equipment. Submerge the glass container in water to eliminate any air. Cut a small piece of white phosphorus and ignite it inside the container. The phosphorus will burn, producing a cold, greenish-white flame due to the presence of phosphorus pentoxide. Remember to wear appropriate safety gear and perform the experiment in a well-ventilated area.
