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How might you use radioactive phosphorus to demonstrate that transforming compound of bacteria in Griffiths experiment was DNA?
In Griffith's experiment, the transforming principle was identified as DNA. To demonstrate this using radioactive phosphorus, one could label DNA with phosphorus-32, as DNA contains phosphorus in its backbone, while proteins do not. By introducing the radioactive DNA into non-virulent bacteria and observing the transformation into virulent forms, one could track the incorporation of the radioactive label. If the transformed bacteria contained radioactivity, it would indicate that DNA was the transforming material, supporting the conclusion that DNA carries genetic information.
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
What of the three experiments gave evidence that protein was not the genetic material?
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
After 3 replication cycles, 87.5% of the DNA will be radioactive. Each cycle doubles the amount of DNA, so after the first cycle, 50% will be radioactive, after the second cycle, 75% will be radioactive, and after the third cycle, 87.5% will be radioactive.
Why do plants need phosphorus?
Plants need phosphorus to support various essential functions, such as photosynthesis, energy transfer, and DNA synthesis. Phosphorus is a key component of ATP, which is the main energy source for cellular processes in plants. Additionally, phosphorus is important for root development and overall plant growth.
What happens when T2 phages are grown in the presence of radioactive phosphorus?
When T2 phages are grown in the presence of radioactive phosphorus, the phage DNA will incorporate the radioactive phosphorus into its structure during replication. This allows for visualizing the localization of the phage DNA within the infected bacterial cell using autoradiography.
Which molecule for which phosphorus-32 is used as a radioactive marker?
Dna
Why did Hershey and chase label the viral DNA with radioactive phosphorus and not radioactive sulfur?
Hershey and Chase labeled the viral DNA with radioactive phosphorus because DNA contains phosphorus in its backbone, while proteins contain sulfur in their amino acids. By using radioactive phosphorus, they could specifically track the DNA of the virus and determine whether it was the genetic material responsible for infection. In contrast, labeling with sulfur would have risked confusion, as it would track the viral proteins instead of the DNA. This choice was crucial for demonstrating that DNA, not protein, was the hereditary material in viruses.
How might you use radioactive phosphorus to demonstrate that transforming compound of bacteria in Griffiths experiment was DNA?
In Griffith's experiment, the transforming principle was identified as DNA. To demonstrate this using radioactive phosphorus, one could label DNA with phosphorus-32, as DNA contains phosphorus in its backbone, while proteins do not. By introducing the radioactive DNA into non-virulent bacteria and observing the transformation into virulent forms, one could track the incorporation of the radioactive label. If the transformed bacteria contained radioactivity, it would indicate that DNA was the transforming material, supporting the conclusion that DNA carries genetic information.
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
What is the difference between phosphorus 31p and 32p?
Phosphorus-31 (31P) is a stable isotope of phosphorus that is commonly used in nuclear magnetic resonance (NMR) spectroscopy, while phosphorus-32 (32P) is a radioactive isotope of phosphorus commonly used in biological research for labeling and tracking DNA and proteins. The main difference is the stability of the isotopes, with 31P being stable and 32P being radioactive.
What of the three experiments gave evidence that protein was not the genetic material?
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
What did Hershey and chase discover using radioactive labeling?
Hershey and Chase discovered that DNA, not protein, is the genetic material responsible for heredity. In their famous experiment with bacteriophages, they used radioactive labeling to distinguish between DNA and protein: they labeled DNA with phosphorus-32 and protein with sulfur-35. After allowing the viruses to infect bacteria, they found that only the radioactive DNA entered the bacterial cells, confirming that DNA carries the genetic instructions for the production of new viruses. This landmark discovery significantly advanced the understanding of genetics.
What are two advantages of phosphorus?
Phosphorus is essential for healthy plant growth as it plays a key role in photosynthesis and energy transfer within the plant. Additionally, phosphorus is important in the formation of DNA, RNA, and ATP in living organisms.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
After 3 replication cycles, 87.5% of the DNA will be radioactive. Each cycle doubles the amount of DNA, so after the first cycle, 50% will be radioactive, after the second cycle, 75% will be radioactive, and after the third cycle, 87.5% will be radioactive.
When was Integrated DNA Technologies created?
Integrated DNA Technologies was created in 1987.
