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How might you use radioactive phosphorus to demonstrate that transforming compound of bacteria in Griffiths experiment was DNA?
In Griffith's experiment, the transforming principle was identified as DNA. To demonstrate this using radioactive phosphorus, one could label DNA with phosphorus-32, as DNA contains phosphorus in its backbone, while proteins do not. By introducing the radioactive DNA into non-virulent bacteria and observing the transformation into virulent forms, one could track the incorporation of the radioactive label. If the transformed bacteria contained radioactivity, it would indicate that DNA was the transforming material, supporting the conclusion that DNA carries genetic information.
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
After 3 replication cycles, 87.5% of the DNA will be radioactive. Each cycle doubles the amount of DNA, so after the first cycle, 50% will be radioactive, after the second cycle, 75% will be radioactive, and after the third cycle, 87.5% will be radioactive.
Why do plants need phosphorus?
Plants need phosphorus to support various essential functions, such as photosynthesis, energy transfer, and DNA synthesis. Phosphorus is a key component of ATP, which is the main energy source for cellular processes in plants. Additionally, phosphorus is important for root development and overall plant growth.
Why did Hershey and Chase grow viruses in cultures that contained both radioactive Sulfur. What might have happened if they had used only one radioactive substance.?
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
What happens when T2 phages are grown in the presence of radioactive phosphorus?
When T2 phages are grown in the presence of radioactive phosphorus, the phage DNA will incorporate the radioactive phosphorus into its structure during replication. This allows for visualizing the localization of the phage DNA within the infected bacterial cell using autoradiography.
Which molecule for which phosphorus-32 is used as a radioactive marker?
Dna
How might you use radioactive phosphorus to demonstrate that transforming compound of bacteria in Griffiths experiment was DNA?
In Griffith's experiment, the transforming principle was identified as DNA. To demonstrate this using radioactive phosphorus, one could label DNA with phosphorus-32, as DNA contains phosphorus in its backbone, while proteins do not. By introducing the radioactive DNA into non-virulent bacteria and observing the transformation into virulent forms, one could track the incorporation of the radioactive label. If the transformed bacteria contained radioactivity, it would indicate that DNA was the transforming material, supporting the conclusion that DNA carries genetic information.
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
What is the difference between phosphorus 31p and 32p?
Phosphorus-31 (31P) is a stable isotope of phosphorus that is commonly used in nuclear magnetic resonance (NMR) spectroscopy, while phosphorus-32 (32P) is a radioactive isotope of phosphorus commonly used in biological research for labeling and tracking DNA and proteins. The main difference is the stability of the isotopes, with 31P being stable and 32P being radioactive.
What are two advantages of phosphorus?
Phosphorus is essential for healthy plant growth as it plays a key role in photosynthesis and energy transfer within the plant. Additionally, phosphorus is important in the formation of DNA, RNA, and ATP in living organisms.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
After 3 replication cycles, 87.5% of the DNA will be radioactive. Each cycle doubles the amount of DNA, so after the first cycle, 50% will be radioactive, after the second cycle, 75% will be radioactive, and after the third cycle, 87.5% will be radioactive.
When was Integrated DNA Technologies created?
Integrated DNA Technologies was created in 1987.
How did Hershey and chase show that DNA was the molecule that carried the genetic code?
Hershey (you know, Alfred Hershy) and Chase (you know again, Martha Chase) did some experiments with Bacteriophages (you know, viruses that affect Bacteria). What they did in the experiment was that, they grew some of these Bacteriophages on a medium which contained radioactive Sulphus(S)(remember, radioactivity can be detected easily). Then, they also took some other Bacteriophages. But, they were grown on radioactive Phosphorur(P)..... (Don't 4get(that's forget)), DNA also has Phosphorus in it (DNA = Nitrogen bases + Pentose sugar + Phosphorus). Now, back to the experiment ------ The Bacteriophages that were grown on radioactive Phosphorus contained radioactive DNA. But they didn't have any radioactive protein coat. (remember viruses have a protein coat covering their DNA?) Then, bacteriophages (please call them B from now, but not in the exams), which were grown in S (radioactive sulphur, remember?), had a radioactive protein coat (protien coats are made up of sulphur and some other elements), but no radioactive DNA........ That was the first part....................... Now, the next one.......................... Radioactive B's, from both P and S were allowed to attach to E.coli (Esterichia coli) bacteria as an attack (or infection). After infection, The protein coats were removed by agitating (stirring real fast) in a blender (they're still not removed). Then, these virus particles (protein coats) wer removed by centrifugation (using centrifugal machine). Then, the third big step....................................… The observation part....................................… It was observed that, some bacteria were radioactive and many were not. Also, it was observed that, bacteria which were attacked by B (Bacteriophage) having radioactive DNA were the ones which were radioactive..... Also that, bacteria which were attacked by B having S weren't radioactive...... So, it lead to the point that proteins didn't pass into bacteria, but it was the DNA that did...... So, DNA was concluded to be the genetic material...... Viruses were chosen because they only had genetic meterial & coat This is from yahoo anwsers btw
What is made out of phosphorus?
Phosphorus is commonly used in fertilizers, detergents, and matches. It is also used in the production of foods, beverages, and metal alloys. Additionally, phosphorus compounds are found in DNA and RNA, playing a crucial role in biological processes.
Why do plants need phosphorus?
Plants need phosphorus to support various essential functions, such as photosynthesis, energy transfer, and DNA synthesis. Phosphorus is a key component of ATP, which is the main energy source for cellular processes in plants. Additionally, phosphorus is important for root development and overall plant growth.
