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If you have a small gene that you wish replicated by pcr you add radioactively labeled nucleotides to the pcr machine after 3 replication cycles how many double-stranded DNA molecules do you have?
After 3 replication cycles in PCR, the number of double-stranded DNA molecules doubles each cycle. Therefore, after 3 cycles, you would have 8 double-stranded DNA molecules.
What does DNA replication make?
DNA replication produces a copy of the DNA. At the same time the cell in which the DNA is to be found splits into two with a copy of the DNA in each. DNA replication is caused by cell replication during the process of mitosis.
What process makes a copy of a DNA molecule?
Transcription.
What is the site of DNA replication in eukaryotes?
The site of DNA replication in eukaryotes is the nucleus. Replication occurs in the nucleus because this is where the DNA is stored. The process involves unwinding the DNA double helix and synthesizing new strands of DNA using the existing strands as templates.
IF DNA is labeled radioactive and allowed to replicate how much radioactivity in newly synthesized DNA?
The amount of radioactivity in the newly synthesized DNA will be equal to that of the original labeled DNA. Since the labeled DNA is used as a template for replication, the radioactivity is retained in the newly synthesized strands.
If you have a small gene that you wish replicated by pcr you add radioactively labeled nucleotides to the pcr machine after 3 replication cycles how many double-stranded DNA molecules do you have?
After 3 replication cycles in PCR, the number of double-stranded DNA molecules doubles each cycle. Therefore, after 3 cycles, you would have 8 double-stranded DNA molecules.
If scientists introduced radioactive thymidine into the growth medium of the cells when would it be incorporated into the DNA molecule?
Radioactive thymidine would be incorporated into the DNA molecule during the S phase of the cell cycle, specifically during DNA replication when new DNA is synthesized. Thymidine is a nucleotide base that is used to build DNA, so introducing radioactive thymidine would result in the incorporation of radioactive material into the newly synthesized DNA strands.
What is a a cause of DNA mutation?
Background radiation, radioactive atoms within DNA strands, replication errors, and random events including chemical "mistakes".
What are the areas on DNA where replication occurs called?
DNA replication begins in areas of DNA molecules are called origins of replication.
Compare the number of replication forks in prokaryotic and eukaryotic DNA during replication?
Prokaryotic DNA replication has a single origin of replication, leading to two replication forks. In contrast, eukaryotic DNA replication has multiple origins of replication, resulting in multiple replication forks forming along the DNA molecule.
What happens when T2 phages are grown in the presence of radioactive phosphorus?
When T2 phages are grown in the presence of radioactive phosphorus, the phage DNA will incorporate the radioactive phosphorus into its structure during replication. This allows for visualizing the localization of the phage DNA within the infected bacterial cell using autoradiography.
DNA is copied during a process called?
DNA is copied during a process called DNA replication. This process occurs in the nucleus of a cell and involves making an exact copy of the original DNA molecule. DNA replication is essential for cell division and passing genetic information from one generation to the next.
What does DNA replication make?
DNA replication produces a copy of the DNA. At the same time the cell in which the DNA is to be found splits into two with a copy of the DNA in each. DNA replication is caused by cell replication during the process of mitosis.
What process makes a copy of a DNA molecule?
Transcription.
What are two ways to classify viruses?
Viruses can be classified based on their structure (DNA or RNA, enveloped or non-enveloped) and their method of replication (lytic or lysogenic life cycles).
Where does DNA polymerase add nucleotides during the process of DNA replication?
DNA polymerase adds nucleotides to the growing DNA strand at the replication fork during the process of DNA replication.
What is the site of DNA replication in eukaryotes?
The site of DNA replication in eukaryotes is the nucleus. Replication occurs in the nucleus because this is where the DNA is stored. The process involves unwinding the DNA double helix and synthesizing new strands of DNA using the existing strands as templates.
