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How is barfoed reagent able to distinguish a reducing monosaccharide from a reducing disaccharide?

Barfoed reagent contains copper ions that can react with reducing sugars to form a brick-red precipitate. Since monosaccharides have a simpler structure and can react more quickly than disaccharides, they will produce a positive result for the test within a shorter period. This allows Barfoed reagent to distinguish between them based on the rate of reaction and appearance of the precipitate.


Why did the enzyme react to lactose but not to sucrose?

the enzyme is not the right shape for sucrose


How would lactose react towards benedict's?

Lactose is a disaccharide composed of glucose and galactose, therefore it would react with Benedict's reagent to produce a positive result. Benedict's reagent is used to test for reducing sugars, and since lactose contains a free anomeric carbon that can reduce copper ions present in the reagent, it would give a color change from blue to brick-red precipitate upon heating if lactose is present.


What is difference between lactic acid and lactose?

If we are talking about distinguishing between the two solutions then, Carry out a Benedict's test on both solutions; Results: Lactose solution would give a brick-red/brown precipitate (positive test) Sucrose solution would give negative test result while sucrose is a non-reducing sugar which does not react with Benedict's reagent.


If a solution has a pH of 5 what would it react with to form a salt?

A solution with a pH of 5 would react with a base to form a salt. The base would neutralize the acidic solution, resulting in the formation of a salt and water.

Related Questions

How would lactose react toward barford test?

Theoretically nothing is happend


How is barfoed reagent able to distinguish a reducing monosaccharide from a reducing disaccharide?

Barfoed reagent contains copper ions that can react with reducing sugars to form a brick-red precipitate. Since monosaccharides have a simpler structure and can react more quickly than disaccharides, they will produce a positive result for the test within a shorter period. This allows Barfoed reagent to distinguish between them based on the rate of reaction and appearance of the precipitate.


Difference between Benedict's test and barfoed's test?

Barfoed's test is used for detecting the presence of monosaccharides.It is based on the reduction of copper(II) (as acetate) to copper(I) oxide (Cu2O), which forms a brick-red precipitate. (Disaccharides may also react, but the reaction is much slower.)Composition:Barfoed's reagent consists of a 0.33 molar solution of neutral copper acetate in 1% acetic acid solution. The reagent does not keep well and it is therefore advisable to make it up when it is actually required.The aldehyde group of the monosaccharide which normally forms a cyclic hemiacetal is oxidized to the carboxylate. A number of other substances, including sodium chloride may interfere.The test is similar to the reaction of Fehling's solution to aldehydes or Benedict's test.There is no difference for a solution to be tested by Benedict`s or by Barfoed`s test, as both detect the presence of reducing sugars. Barfoed`s test is the least known of these three.


How are the condition of barfoed's test different from those of nenedict's test?

Barfoed's test is used to distinguish monosaccharides from disaccharides, as it requires a monosaccharide to react within a few minutes. On the other hand, Benedict's test is used to detect the presence of reducing sugars, including both monosaccharides and some disaccharides, by forming a colored precipitate. Benedict's test typically takes longer to develop a positive result compared to Barfoed's test.


Why did the enzyme react to lactose but not to sucrose?

the enzyme is not the right shape for sucrose


How would lactose react towards benedict's?

Lactose is a disaccharide composed of glucose and galactose, therefore it would react with Benedict's reagent to produce a positive result. Benedict's reagent is used to test for reducing sugars, and since lactose contains a free anomeric carbon that can reduce copper ions present in the reagent, it would give a color change from blue to brick-red precipitate upon heating if lactose is present.


Will Silver metal react with a solution of copper sulfate and vice versa?

They do not react.


How does phosphorus react with aqueous cuso4?

Phosphorus does not react with aqueous CuSO4. Copper sulfate solution is a salt solution and does not react with phosphorus to produce any significant chemical reaction.


How do aldehyde's react?

aldehyde is less stable than benzene so it react readily toward kmno4


Does lemon juice react in benedict's solution?

no


What does salt react with?

Salt can react with water to form a solution called a saline solution. It can also react with certain metals, such as iron, to cause corrosion. Additionally, salt can react with acids and bases to form different compounds.


If you add more of a solution to a quantity of water will it react?

It may or may not, if it's supposed to react it probably will, unless you added too much of your solution.