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Enterococci and Streptococci are separated into Lancefield groups based on the presence or absence of specific antigens on their cell walls. This classification is determined through serological testing, such as the Lancefield grouping test, which detects the presence of specific carbohydrates in the bacterial cell wall. By identifying these antigens, bacteria can be categorized into different Lancefield groups, which aids in distinguishing between various species and strains.

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