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What else can I help you with?
Is a non motile organism over inoculation of the slide with organisms a false negative?
If a slide is over-inoculated, it means that there are too many samples on one slide. This means that it can give a false negative regarding the motility of the organism in question.
Why is MacConkey inoculated with a urine specimen?
MacConkey agar is selective for Gram-negative bacteria and can differentiate lactose fermenters from non-fermenters based on color change. Urine samples typically contain a mixture of bacteria, including potential pathogens that might be inhibited on other media. Inoculating MacConkey agar allows for the isolation and identification of Gram-negative bacteria, particularly Enterobacteriaceae, which are common urinary tract pathogens.
What is the negative control in a fermentation lab?
Howdy there! Negative control for a fermentation lab should have the substrate in the vessel, and it should be very sterile so no contamination doesn't happen (make sure to label them vessels well). Secondly, the negative control vessel should not be inoculated with the microbe, like yeast, in it at all because you want no response. If there is a response, it could be contamination or you mistakenly inoculated with the microbe which fermented the substrate. Hope this helped you plenty! 😄
What selective growth media is best suited for isolation of coliform bacteria?
MacConkey's agar is a selective growth media that is best suited for isolation of coliform bacteria. This bacterial is red and is gram negative. The agar plate inhibits the growth of gram positive organisms and allows gram negative organisms to grow.
What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
Why 7.5 percent sodium chloride agar is selective media?
Because 7.5% NaCl agar inhibits gram negative organisms from growing, and is "selective" in that only gram positive organisms can grow in this type of agar.
Is a non motile organism over inoculation of the slide with organisms a false negative?
If a slide is over-inoculated, it means that there are too many samples on one slide. This means that it can give a false negative regarding the motility of the organism in question.
Do Gram negative organisms grow on MSA plates?
No, Gram-negative organisms do not grow on Mannitol Salt Agar (MSA) plates because MSA contains high salt concentration and phenol red, which create a selective and differential medium that inhibits the growth of most Gram-negative bacteria while allowing for the growth of certain Gram-positive bacteria.
Can selective connotations be negative?
Yes, selective connotations can be negative if they involve the deliberate highlighting of certain negative meanings or interpretations associated with a particular word or phrase while ignoring other more neutral or positive connotations. This can lead to bias and misrepresentation.
Why is MacConkey inoculated with a urine specimen?
MacConkey agar is selective for Gram-negative bacteria and can differentiate lactose fermenters from non-fermenters based on color change. Urine samples typically contain a mixture of bacteria, including potential pathogens that might be inhibited on other media. Inoculating MacConkey agar allows for the isolation and identification of Gram-negative bacteria, particularly Enterobacteriaceae, which are common urinary tract pathogens.
What is the negative control in a fermentation lab?
Howdy there! Negative control for a fermentation lab should have the substrate in the vessel, and it should be very sterile so no contamination doesn't happen (make sure to label them vessels well). Secondly, the negative control vessel should not be inoculated with the microbe, like yeast, in it at all because you want no response. If there is a response, it could be contamination or you mistakenly inoculated with the microbe which fermented the substrate. Hope this helped you plenty! 😄
Does the bacillus cereus growth on Phenylethyl Alcohol agar?
Yes, Bacillus cereus can grow on Phenylethyl Alcohol agar as it is a selective medium used for isolating Gram-positive organisms including Bacillus species. The agar contains phenylethyl alcohol which inhibits the growth of Gram-negative bacteria, allowing for the selective growth of Gram-positive bacteria like Bacillus cereus.
What could be a selective medium in eliminating gram positive and gram negative from a mixture?
streptococ
Is phenylethyl alcohol agar differentia or selective?
Phenylethyl alcohol agar is a selective medium used for the isolation of gram-positive bacteria as it inhibits the growth of gram-negative bacteria. It is not typically used for differentiation purposes.
What selective growth media is best suited for isolation of coliform bacteria?
MacConkey's agar is a selective growth media that is best suited for isolation of coliform bacteria. This bacterial is red and is gram negative. The agar plate inhibits the growth of gram positive organisms and allows gram negative organisms to grow.
List selective media for stool culture?
MacConkey agar: Selective for Gram-negative bacteria, inhibits the growth of Gram-positive bacteria. Hektoen enteric agar: Selective for Gram-negative enteric pathogens, such as Salmonella and Shigella species. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar: Selective for Vibrio species, especially Vibrio cholerae. Cefsulodin-irgasan-novobiocin (CIN) agar: Selective for Yersinia species.
Is glucose salts agar selective?
Glucose Salts Agar (GSA) is a selective agar that selects for gram-negative bacteria only. This means that no gram-positive bacteria will be able to grow on it.
