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Does the bacillus cereus growth on Phenylethyl Alcohol agar?
Yes, Bacillus cereus can grow on Phenylethyl Alcohol agar as it is a selective medium used for isolating Gram-positive organisms including Bacillus species. The agar contains phenylethyl alcohol which inhibits the growth of Gram-negative bacteria, allowing for the selective growth of Gram-positive bacteria like Bacillus cereus.
What is phenyl ethyl alcohol agar?
Phenylethyl alcohol agar (PEA) is a selective media used to cultivate Gram (+)microorganisms. The active ingredient, phenylethyl alcohol, inhibits or reduces growth of Gram (-) microorganisms by interfering with DNA synthesis.
Which ingredient in a phenylethyl alcohol agar supplies carbon and nitrogen?
Peptone in phenylethyl alcohol agar supplies both carbon and nitrogen to support the growth of microorganisms. Peptone is a mixture of partially digested proteins that serve as a nutrient source for microbial growth.
What is the composition of PEA agar?
Phenylethyl Alcohol Agar (PEA) is prepared using the following incredients:Pancreatic Enzyme digest of Casein.......15.0gPapaic digest of Soybean Meal.................5.0gSodium Chloride.....................................5.0gYeast Extract..........................................5.0gPhenylethanol........................................2.5gVitamin K..........................................10.0mgHemin.................................................5.0mgL-Cystine.............................................0.4mgSheep Blood........................................50.0mlAgar.....................................................15.0gComponents are mixed in 1 L water. If making less than 1 L media, change components accordingly. Media should be prepared at pH 7.3 +/- 0.2. After mixing components and adjusting pH, autoclave at 121 degrees C for 20 min then placed into water bath at 50 degrees C to allow it to cool enough to be poured.
List selective media for stool culture?
MacConkey agar: Selective for Gram-negative bacteria, inhibits the growth of Gram-positive bacteria. Hektoen enteric agar: Selective for Gram-negative enteric pathogens, such as Salmonella and Shigella species. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar: Selective for Vibrio species, especially Vibrio cholerae. Cefsulodin-irgasan-novobiocin (CIN) agar: Selective for Yersinia species.
Does the bacillus cereus growth on Phenylethyl Alcohol agar?
Yes, Bacillus cereus can grow on Phenylethyl Alcohol agar as it is a selective medium used for isolating Gram-positive organisms including Bacillus species. The agar contains phenylethyl alcohol which inhibits the growth of Gram-negative bacteria, allowing for the selective growth of Gram-positive bacteria like Bacillus cereus.
What is phenyl ethyl alcohol agar?
Phenylethyl alcohol agar (PEA) is a selective media used to cultivate Gram (+)microorganisms. The active ingredient, phenylethyl alcohol, inhibits or reduces growth of Gram (-) microorganisms by interfering with DNA synthesis.
Would removing phenylethyl alcohol from PEA alter the medium and sensitivity or specificity?
Removing phenylethyl alcohol from PEA (Phenylethyl Alcohol Agar) would likely alter the medium's effectiveness in inhibiting the growth of gram-negative bacteria while allowing gram-positive bacteria to thrive. This could lead to decreased sensitivity for the detection of certain organisms, as the selective pressure provided by phenylethyl alcohol would be lost. Consequently, the specificity of the medium might also be affected, potentially allowing for the growth of unwanted gram-negative organisms that would normally be suppressed.
Which ingredient in a phenylethyl alcohol agar supplies carbon and nitrogen?
Peptone in phenylethyl alcohol agar supplies both carbon and nitrogen to support the growth of microorganisms. Peptone is a mixture of partially digested proteins that serve as a nutrient source for microbial growth.
What ingredient in PEA supplies nitrogen?
PEA agar, also referred to as Phenylethyl Alcohol Agar is a medium used by microbiologists to grow microorganisms. The ingredient that supplies nitrogen is casein.
What constitutes good growth on phenylethyl alcohol agar plate?
Good growth on phenylethyl alcohol (PEA) agar is characterized by the presence of well-defined, opaque colonies. The medium inhibits the growth of Gram-negative bacteria, so the expected growth mainly comprises Gram-positive organisms, often appearing creamy, white, or yellowish. Additionally, the colonies should be evenly distributed and not overly wet or mucoid, indicating healthy growth conditions. Overall, good growth reflects the organism's ability to thrive in the selective environment provided by PEA agar.
Which ingredient in phenylethyl alcohol agar supplies nitrogen?
The ingredient in phenylethyl alcohol agar that supplies nitrogen is peptone. Peptone is a protein digest that provides essential amino acids and nitrogen sources for the growth of certain bacteria. This makes it a crucial component for supporting the growth of organisms while inhibiting the growth of gram-negative bacteria in the medium.
If you observed growth of gram negative organisms on the PEA plate you inoculated does the negate the usefulness of PEA as a selective medium why or why not?
Yes, the growth of gram-negative organisms on a PEA plate suggests that the medium is not effectively selective for gram-positive bacteria. PEA (Phenylethyl Alcohol Agar) is intended to inhibit gram-negative bacteria through the action of phenylethyl alcohol, so if gram-negative organisms are thriving, it indicates that their growth is not adequately suppressed. This could be due to several factors, including the specific strains or concentrations used, which may indicate limitations in PEA's selectivity under certain conditions.
What is the composition of PEA agar?
Phenylethyl Alcohol Agar (PEA) is prepared using the following incredients:Pancreatic Enzyme digest of Casein.......15.0gPapaic digest of Soybean Meal.................5.0gSodium Chloride.....................................5.0gYeast Extract..........................................5.0gPhenylethanol........................................2.5gVitamin K..........................................10.0mgHemin.................................................5.0mgL-Cystine.............................................0.4mgSheep Blood........................................50.0mlAgar.....................................................15.0gComponents are mixed in 1 L water. If making less than 1 L media, change components accordingly. Media should be prepared at pH 7.3 +/- 0.2. After mixing components and adjusting pH, autoclave at 121 degrees C for 20 min then placed into water bath at 50 degrees C to allow it to cool enough to be poured.
Is a tsa agar selective or differential?
its a selective media
List selective media for stool culture?
MacConkey agar: Selective for Gram-negative bacteria, inhibits the growth of Gram-positive bacteria. Hektoen enteric agar: Selective for Gram-negative enteric pathogens, such as Salmonella and Shigella species. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar: Selective for Vibrio species, especially Vibrio cholerae. Cefsulodin-irgasan-novobiocin (CIN) agar: Selective for Yersinia species.
Which ingredient in PEA supplies carbon?
Casein is the ingredient in PEA that supplies carbon, as well as nitrogen. PEA is also known as Phenylethyl Alcohol Blood Agar.
