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5.0g and 5.00g are different because they contain different numbers of significant figures. 5.0 grams means that the measurement or value is correct within +- 0.1g. On the other hand, 5.00 means that the value is correct within +-0.01g.

5.00 is more accurate than 5.0, because it goes to the 100ths. Engineering wise you round up numbers to the 10ths

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