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  1. Deparaffinize and hydrate tissue sections.
  2. Stain with a 1% solution of potassium permanganate for 5 minutes.
  3. Rinse with tap water.
  4. Counterstain with a 1% solution of oxalic acid for 1-2 minutes.
  5. Rinse and dehydrate before mounting with a coverslip.
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What stain would be used for reading a retic?

A reticulin stain would be used to highlight reticular fibers in tissues, particularly in histology studies of organs like the liver or bone marrow. It is commonly used to demonstrate the presence of these fine, delicate fibers that form a supportive network in certain tissues.


What is the importance of hematoxylin stain in histology?

Hematoxylin stain is used in histology to selectively stain cell nuclei blue, allowing for better visualization of tissue morphology and structures. It serves as a contrast stain in combination with eosin stain, highlighting cellular and tissue features for microscopic analysis and diagnosis.


What is a histology special stain?

A histology special stain is a type of staining technique used in histology to selectively highlight specific structures or substances in tissue samples that may not be visible with standard staining methods. These stains help in visualizing different components of cells or tissues, aiding in the identification and diagnosis of various diseases.


What histology stain should be used for brain tissue?

The most common histology stain used for brain tissue is hematoxylin and eosin (H&E) staining. This stain allows for visualization of the overall tissue architecture, cell morphology, and distinguishing between different cell types in the brain. Other stains like Nissl stain can be used to highlight neuronal cell bodies and their distribution in the brain.


What is the gram stain of enterobacter cloacae?

Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.

Related Questions

What stain would be used for reading a retic?

A reticulin stain would be used to highlight reticular fibers in tissues, particularly in histology studies of organs like the liver or bone marrow. It is commonly used to demonstrate the presence of these fine, delicate fibers that form a supportive network in certain tissues.


What is the importance of hematoxylin stain in histology?

Hematoxylin stain is used in histology to selectively stain cell nuclei blue, allowing for better visualization of tissue morphology and structures. It serves as a contrast stain in combination with eosin stain, highlighting cellular and tissue features for microscopic analysis and diagnosis.


What is the counter or secondary stain used in the Gram stain procedure?

The counter or secondary stain used in the Gram stain procedure is safranin.


What is a histology special stain?

A histology special stain is a type of staining technique used in histology to selectively highlight specific structures or substances in tissue samples that may not be visible with standard staining methods. These stains help in visualizing different components of cells or tissues, aiding in the identification and diagnosis of various diseases.


What is safranin solution in science?

safranin is a biological stain used in histology n cytology


What is the purpose of the crystal violet in the gram stain procedure?

Its the primary stain of the procedure. IT stains the Gram positive organisms


How long does a gram stain procedure typically take?

A gram stain procedure typically takes about 10-15 minutes to complete.


Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?

The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).


What histology stain should be used for brain tissue?

The most common histology stain used for brain tissue is hematoxylin and eosin (H&E) staining. This stain allows for visualization of the overall tissue architecture, cell morphology, and distinguishing between different cell types in the brain. Other stains like Nissl stain can be used to highlight neuronal cell bodies and their distribution in the brain.


What is the gram stain of enterobacter cloacae?

Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.


What is the appearance of capsule after being stain with capsule stain procedure?

Capsules appear as a clear halo surrounding stained bacteria when using the capsule stain procedure. The capsule itself does not stain, allowing it to show up as a clear area against the stained background of the bacteria.


Does the gram stain procedure work for all bacteria?

Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.