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Pepsin is an enzyme that catalyzes the breakdown of proteins into polypeptides and amino acids through hydrolysis. In DNA extraction, this proteolytic processing is essential in dissolving the cellular and histone proteins that bind the DNA strands.

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Which part of the cell contains the code for pepsin?

The code for pepsin is contained in the cell's nucleus in the form of DNA. This DNA is transcribed into mRNA, which is then translated into protein in the cell's cytoplasm.


Why we can not extract DNA from RBCs?

We can not extract DNA from RBCs as they are without nucleus. only the source of DNA extraction is Leukocytes, RBCs are not good source of extraction but we can extract DNA from immature RBCs.


What is the purpose of incubation in DNA extraction?

Incubation in DNA extraction allows the DNA to be released from cells by breaking down cell membranes and proteins. This process helps separate the DNA from other cellular components, making it easier to isolate and purify the DNA for downstream applications such as PCR or sequencing.


Why is it difficult to extract DNA from a cell fat tissue?

It is difficult to extract DNA from fat tissue because of the high lipid content in the cells. Lipids can interfere with the DNA extraction process by binding to the DNA and preventing it from being isolated effectively. Specialized protocols and reagents are needed to remove the lipids and extract the DNA successfully from fat tissue.


What is the purpose of adding isopropyl alcohol to your sample of DNA?

Isopropyl alcohol is added to DNA samples to precipitate the DNA, facilitating its separation from the aqueous solution. When isopropyl alcohol is mixed with a DNA solution, it causes the DNA to become less soluble, allowing it to clump together and form visible strands. This process enhances the yield and purity of the isolated DNA, making it easier to extract for further analysis or experimentation.

Related Questions

Why cant you see DNA within a DNA?

Because the purpose of this lab is to extract DNA from a variety of cells and see DNA


Which part of the cell contains the code for pepsin?

The code for pepsin is contained in the cell's nucleus in the form of DNA. This DNA is transcribed into mRNA, which is then translated into protein in the cell's cytoplasm.


What is the purpose of an extract?

An extract is a passage


Can you extract DNA from an onion?

si


Can you extract DNA from fruit?

Yes.


Can extract DNA from fruit?

Yes.


Can you extract DNA from ear wax?

Yes, it is possible to extract DNA from ear wax as it contains skin cells that can be used for genetic testing.


Can you extract DNA from sweat?

Yes, it is possible to extract DNA from sweat. Sweat contains skin cells that may contain DNA, which can be extracted using specialized techniques for analysis and identification.


Is it possible to extract DNA from blood samples?

Yes, it is possible to extract DNA from blood samples. This process involves isolating the DNA molecules from the blood cells and purifying them for analysis or testing.


Why we can not extract DNA from RBCs?

We can not extract DNA from RBCs as they are without nucleus. only the source of DNA extraction is Leukocytes, RBCs are not good source of extraction but we can extract DNA from immature RBCs.


Why pepsin is inactive in small intestine?

pepsin and all other digestive enzymes are neutralized by the small intestine


What is the purpose of incubation in DNA extraction?

Incubation in DNA extraction allows the DNA to be released from cells by breaking down cell membranes and proteins. This process helps separate the DNA from other cellular components, making it easier to isolate and purify the DNA for downstream applications such as PCR or sequencing.