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This phenomenon occurs because acid-fast staining detects the presence of mycolic acid in the cell wall of bacteria, which is resistant to decolorization by acid-alcohol. Spores, which have a thick protein coat that is resistant to staining, can sometimes retain the acid-fast stain due to this resistance. Conversely, the resistance of acid-fast bacteria to decolorization can allow spores to be stained if present.

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1y ago

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