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What is a repressible operon?
A repressible operon is a type of operon in bacteria where gene expression is usually active but can be turned off when a specific corepressor molecule binds to the repressor protein. This binding causes the repressor to bind to the operator region of the operon, blocking transcription and thus shutting down gene expression. An example of a repressible operon is the trp operon in E. coli, which is involved in tryptophan biosynthesis.
What state is the repressor protein in when tryptophan is absent?
When tryptophan is absent, the repressor protein is in an inactive state, allowing transcription of the trp operon to continue. This is because the repressor protein needs tryptophan to bind to it, enabling it to attach to the operator region and block transcription of the operon.
How is the ath operon most likely controlled?
The ath operon is most likely controlled by a repressor protein that binds to the operator region to prevent transcription. This repressor protein may undergo conformational changes in response to the presence of aromatic amino acids, allowing for the expression of the operon when these amino acids are scarce.
Why lac operon the gene expression is switch off?
explain the regulation of gene expression in lac operon.
Is operon proteins?
Operon is not a protein. It is a segment of DNA that has cluster of genes controlled by the elements such as promoter, operator. Lac operon is a classic example where it regulate the utilization of Lactose in the medium.
What acts as a co-repressor to block transcription of the tryptophan operon?
The corepressor tryptophan itself binds to the repressor protein, causing a conformational change that allows it to bind to the operator sequence of the tryptophan operon. This blocks RNA polymerase from transcribing the operon, leading to repression of tryptophan biosynthesis.
Why do both bacteria and humans need tryptophan?
Tryptophan induces the lac operon.
What are the roles of allolactose in the lac operon and tryptophan in trp operon?
allolactose acts as an inducer
Why is the tryptophan operon turned off in the presence of tryptophan?
The tryptophan operon is turned off in the presence of tryptophan because tryptophan acts as a corepressor. When tryptophan levels are high, it binds to the trp repressor protein. This trp-repressor complex then binds to the operator region of the operon, preventing RNA polymerase from transcribing the genes involved in tryptophan synthesis.
The tryptophan operon is a repressible operon that is?
regulated by the availability of tryptophan. When tryptophan levels are high, tryptophan acts as a corepressor, binding to the repressor protein, which then binds to the operator, preventing gene transcription. This allows bacteria to conserve energy by only producing tryptophan when needed.
What is a repressible operon?
A repressible operon is a type of operon in bacteria where gene expression is usually active but can be turned off when a specific corepressor molecule binds to the repressor protein. This binding causes the repressor to bind to the operator region of the operon, blocking transcription and thus shutting down gene expression. An example of a repressible operon is the trp operon in E. coli, which is involved in tryptophan biosynthesis.
What is the comparison between lac operon and trp operon?
The lac operon and trp operon are both key regulatory mechanisms in bacteria that control gene expression in response to environmental conditions. The lac operon, which is involved in lactose metabolism, is activated in the presence of lactose and inhibited by glucose, functioning primarily through an inducible system. In contrast, the trp operon regulates tryptophan biosynthesis and operates as a repressible system, being turned off when tryptophan levels are sufficient. Thus, while the lac operon responds to the availability of substrates, the trp operon responds to the abundance of end products.
What does tryptophan bind to when it is present in e. coli?
trp operon
How is lac operon different from trp operon?
1. in lac operon; gene activity is induced when lactose is present in the medium, whereas in case of trp operon, repression of the gene activity takes place in presence of tryptophan in the medium. 2. lac operon spans about 4-6kb...whereas trp operon spans abut 7kb. 3. lac operon helps in the breakdown of lactose into glucose and galactose, to generate energy( catabolic pathway ) in case of trp operon, it helps in the synthesis of enzymes required for the formation of the amino acid Tryptophan( anabolic pathway ). 4. lac operon is an example of positive regulation ; and trp operon is an example of negative regulation. Trp operon is also regulated by other mechanism called attenuation while no such even occurs in lac operon . .
What state is the repressor protein in when tryptophan is absent?
When tryptophan is absent, the repressor protein is in an inactive state, allowing transcription of the trp operon to continue. This is because the repressor protein needs tryptophan to bind to it, enabling it to attach to the operator region and block transcription of the operon.
How is the ath operon most likely controlled?
The ath operon is most likely controlled by a repressor protein that binds to the operator region to prevent transcription. This repressor protein may undergo conformational changes in response to the presence of aromatic amino acids, allowing for the expression of the operon when these amino acids are scarce.
Why lac operon the gene expression is switch off?
explain the regulation of gene expression in lac operon.
