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Theophilus Painter, one of the preeminent cytologists of the early twentieth century, estimated in 1923 that the number was 48. In 1956, improved techniques enabled researchers Joe Hin Tjio and Albert Levan to publish a paper stating that the number was 46. Within a year, Ford and Hamerton provided confirmatory data, reporting the diploid chromosome number in human testicular cells to be 46. See http://www.nature.com/scitable/topicpage/Human-Chromosome-Number-294 for interesting article.

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