The Bradford reagent (Coomassie) is commonly used to detect if a sample contains protein. Coomassie will react with aromatic amino acids (phenylalanine, tyrosine and tryptophan) to turn from a dull red color to a bright blue color. This assay is dependant on the amount of aromatic amino acids present, but works well as a "quick and dirty" indicator of the presence of a protein. The bicinchonic acid assay (BCA assay), while more expensive than the Bradford assay, more accurately detects the presence of the peptide bond present in proteins, so it can be used to not only detect proteins which lack aromatic amino acid residues, but also can be used to more accurately determine the concentration of protein in a sample as not all proteins have the same amount of aromatic amino acids.
Benedict's
Fouchet's reagent typically contains concentrated sulfuric acid and p-dimethylaminobenzaldehyde. It is used for detecting the presence of phosphates in solution through a color change reaction to a blue-colored complex. Caution should be taken when handling Fouchet's reagent due to its corrosive nature.
Nessler's reagent is toxic and can be fatal if ingested in large amounts. It contains chemicals that can cause severe poisoning and damage to internal organs. Ingestion should be avoided, and immediate medical attention is necessary if exposure occurs.
If the cover of a reagent bottle is placed on a table with the bottom down, it can pick up material from the table which would contaminate the contents of the reagent bottle after the cap is put back on the bottle.
After using a reagent, you should securely close the container to prevent spills or contamination. Store the reagent according to the manufacturer's instructions, making sure to label it with the date it was opened and any other relevant information. Dispose of any waste following proper laboratory protocols.
Benedict's
Fouchet's reagent typically contains concentrated sulfuric acid and p-dimethylaminobenzaldehyde. It is used for detecting the presence of phosphates in solution through a color change reaction to a blue-colored complex. Caution should be taken when handling Fouchet's reagent due to its corrosive nature.
The reagent blank should contain everything that the sample contains, except one variable. That variable could be the active ingredient, the enzyme, the substrate, or some other ingredient that is essential to the reaction. If water is added to all the other tubes, it must also be added to the reagent blank.
Nessler's reagent is toxic and can be fatal if ingested in large amounts. It contains chemicals that can cause severe poisoning and damage to internal organs. Ingestion should be avoided, and immediate medical attention is necessary if exposure occurs.
A random sample should be taken from an entire population.
It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
It should reduce the sample error.
If the cover of a reagent bottle is placed on a table with the bottom down, it can pick up material from the table which would contaminate the contents of the reagent bottle after the cap is put back on the bottle.
1. the sample should be representative thus carefully selected. 2. the sample should be adequate thus significant enough.
The sample should be selected randomly.
After using a reagent, you should securely close the container to prevent spills or contamination. Store the reagent according to the manufacturer's instructions, making sure to label it with the date it was opened and any other relevant information. Dispose of any waste following proper laboratory protocols.