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The Bradford reagent (Coomassie) is commonly used to detect if a sample contains protein. Coomassie will react with aromatic amino acids (phenylalanine, tyrosine and tryptophan) to turn from a dull red color to a bright blue color. This assay is dependant on the amount of aromatic amino acids present, but works well as a "quick and dirty" indicator of the presence of a protein. The bicinchonic acid assay (BCA assay), while more expensive than the Bradford assay, more accurately detects the presence of the peptide bond present in proteins, so it can be used to not only detect proteins which lack aromatic amino acid residues, but also can be used to more accurately determine the concentration of protein in a sample as not all proteins have the same amount of aromatic amino acids.
What else can I help you with?
What test should be done to test whether a sample contains glucose?
Benedict's
What is fouchets reagent content?
Fouchet's reagent typically contains concentrated sulfuric acid and p-dimethylaminobenzaldehyde. It is used for detecting the presence of phosphates in solution through a color change reaction to a blue-colored complex. Caution should be taken when handling Fouchet's reagent due to its corrosive nature.
Do people die if they drink nessler's reagent?
Nessler's reagent is toxic and can be fatal if ingested in large amounts. It contains chemicals that can cause severe poisoning and damage to internal organs. Ingestion should be avoided, and immediate medical attention is necessary if exposure occurs.
Why should screw cap of a reagent bottle be placed bottom up on the table?
If the cover of a reagent bottle is placed on a table with the bottom down, it can pick up material from the table which would contaminate the contents of the reagent bottle after the cap is put back on the bottle.
What should you do when you have finished using a reagent?
After using a reagent, you should securely close the container to prevent spills or contamination. Store the reagent according to the manufacturer's instructions, making sure to label it with the date it was opened and any other relevant information. Dispose of any waste following proper laboratory protocols.
What test should be done to test whether a sample contains glucose?
Benedict's
What is fouchets reagent content?
Fouchet's reagent typically contains concentrated sulfuric acid and p-dimethylaminobenzaldehyde. It is used for detecting the presence of phosphates in solution through a color change reaction to a blue-colored complex. Caution should be taken when handling Fouchet's reagent due to its corrosive nature.
Why do we add water to reagent blank?
The reagent blank should contain everything that the sample contains, except one variable. That variable could be the active ingredient, the enzyme, the substrate, or some other ingredient that is essential to the reaction. If water is added to all the other tubes, it must also be added to the reagent blank.
Do people die if they drink nessler's reagent?
Nessler's reagent is toxic and can be fatal if ingested in large amounts. It contains chemicals that can cause severe poisoning and damage to internal organs. Ingestion should be avoided, and immediate medical attention is necessary if exposure occurs.
A random sample should be taken from?
A random sample should be taken from an entire population.
Why egg albumin and casein did not react ninhydrin reagent?
It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.
Will hydrolysed dextrine give positive response to Benedict test?
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
How does increasing the sample size affect the sample error of the mean?
It should reduce the sample error.
Why should screw cap of a reagent bottle be placed bottom up on the table?
If the cover of a reagent bottle is placed on a table with the bottom down, it can pick up material from the table which would contaminate the contents of the reagent bottle after the cap is put back on the bottle.
Characteristics of a good sample?
1. the sample should be representative thus carefully selected. 2. the sample should be adequate thus significant enough.
How do you ensure less sample bias?
The sample should be selected randomly.
What should you do when you have finished using a reagent?
After using a reagent, you should securely close the container to prevent spills or contamination. Store the reagent according to the manufacturer's instructions, making sure to label it with the date it was opened and any other relevant information. Dispose of any waste following proper laboratory protocols.
