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If you mixed albumin with 5 drops of biuret reagent what color do you get?
If you mix albumin with 5 drops of biuret reagent, the resulting color would most likely be a purple hue. This color change occurs due to the presence of peptide bonds in the protein, which react with the biuret reagent to form a colored complex.
Is casein positive to millon's test?
Yes, because casein is one of the protein that makes up milk. And when milk is denatured (by heat, or by any means), the denatured protein is tyrosine-which is the only protein positive for millon's test.
Why doesn't ninhydrin react with polypeptides?
Ninhydrin does react with polypeptides, specifically with the amino group of amino acids present in the polypeptide chain. This reaction leads to the formation of a colored compound that can be used for detection and analysis of amino acids and polypeptides.
Does ninhydrin has flaws?
Yes, ninhydrin has some drawbacks. It can react with other substances present in the sample, leading to false positive results. Additionally, the reaction with ninhydrin is not specific to a particular amino acid, which can limit its application in identifying specific amino acids.
Does tollens reagent react with cyclohexanone?
Yes, Tollens' reagent can react with cyclohexanone. Tollens' reagent is commonly used to test for the presence of aldehydes, including cyclohexanone, by forming a silver mirror when the aldehyde is present.
If you mixed albumin with 5 drops of biuret reagent what color do you get?
If you mix albumin with 5 drops of biuret reagent, the resulting color would most likely be a purple hue. This color change occurs due to the presence of peptide bonds in the protein, which react with the biuret reagent to form a colored complex.
Is egg albumin positive to millon test?
Yes, egg albumin is positive for the Millon's test due to the presence of aromatic amino acids like tyrosine, which react with Millon's reagent to produce a red color.
Is casein positive to millon's test?
Yes, because casein is one of the protein that makes up milk. And when milk is denatured (by heat, or by any means), the denatured protein is tyrosine-which is the only protein positive for millon's test.
Why doesn't ninhydrin react with polypeptides?
Ninhydrin does react with polypeptides, specifically with the amino group of amino acids present in the polypeptide chain. This reaction leads to the formation of a colored compound that can be used for detection and analysis of amino acids and polypeptides.
Does ninhydrin has flaws?
Yes, ninhydrin has some drawbacks. It can react with other substances present in the sample, leading to false positive results. Additionally, the reaction with ninhydrin is not specific to a particular amino acid, which can limit its application in identifying specific amino acids.
THE REAGENT NINHYDRIN WILL REACT AT WHAT pH?
Ninhydrin reacts effectively at a pH range of approximately 4 to 9, with optimal reactivity typically around neutral pH (around 7). At this pH, it can efficiently interact with amino acids and proteins, producing a colored product, usually purple or blue, which is used as a detection method in various biochemical analyses. At extremely acidic or basic pH levels, the reactivity may decrease significantly.
What are the Benedict result for albumen?
The Benedict's test for albumen (albumin) involves adding Benedict's reagent to the sample and heating it. If reducing sugars are present, the solution will change color, typically from blue to green, yellow, or brick-red, depending on the concentration of sugars. However, albumin itself does not react in this test, as it is a protein and does not contain reducing sugars. Therefore, a positive result for reducing sugars would indicate the presence of sugars, not albumin.
Does tollens reagent react with cyclohexanone?
Yes, Tollens' reagent can react with cyclohexanone. Tollens' reagent is commonly used to test for the presence of aldehydes, including cyclohexanone, by forming a silver mirror when the aldehyde is present.
Why Tollens reagent does not react with ketone?
Tollens reagent is a mild oxidizing agent that reacts with aldehydes to produce a silver mirror. Ketones, however, do not have a hydrogen atom bonded to the carbonyl group, making them resistant to oxidation by Tollens reagent. As a result, ketones do not react with Tollens reagent.
What do you call a substance to react with another element?
A "reagent" or "reactant".
Why biuret regent react with single amino acid?
The biuret reagent should not react with a single amino acid. The reagent reacts when there is a peptide bond linking amino acids together. If you are seeing the biuret reagent react in the presence of a single amino acid, then there must be some amino acids that are still linked together.
What are the implications for primary and secondary amines in their reaction with ninhydrin?
Primary amines react with ninhydrin to produce a deep blue-purple color due to the formation of a colored complex, which is useful for detecting amino acids and peptides. Secondary amines also react, but typically yield a less intense color compared to primary amines. This difference in reactivity can be utilized in analytical chemistry to distinguish between primary and secondary amines in various samples. Tertiary amines do not react with ninhydrin, highlighting the specificity of this reaction in identifying amine types.
