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What is the pH for ferric ammonium citrate?
Ferric ammonium citrate typically has a pH range of around 3.0 to 6.0 when dissolved in water, depending on its concentration and the specific formulation. This acidity is due to the presence of ferric ions and ammonium ions, which can release protons in solution. The exact pH may vary based on the specific product and its preparation method.
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
What are some uses of ammonium citrate?
Ammonium Citrate in distilled water is an alternate developer for Platinum and Palladium photographic prints (Iron replacement by Pt and/or Pd). It is cooler in resulting print color than the usual developer - Potassium Oxalate. See www.bostick-sullivan.com
How does klebsiella pneumoniae use citrate as a source of carbon?
Klebsiella pneumoniae is a member of the Enterobacteriaceae family and is able to anaerobically use citrate as a source of Carbon because the citrate contains citritase, an enzyme that initiates a catabolic process. The carbonate and nitrogen of the citrate is broken down into acetate and oxalacetic acid, and then pyruvic acid and carbon dioxide. These products reacts with the ammonia (nitrogen and hydrogen) and sodium hydrogen carbonate compounds of the Citrate. The K. pneumoniaeaccumulates the alkalines and create a basic pH. (I think?)
In the citrate test explain how an organsm that possesses the citrate lyase enzyme might not test positive on simmos citrate agar is thia a false negative why?
An organism that possesses the citrate lyase enzyme may not test positive on Simmon's citrate agar if it does not effectively utilize citrate under the specific conditions of the test, such as pH or temperature. Additionally, the organism might not be able to grow on the agar due to other metabolic limitations or competition with other microorganisms. This situation is not strictly a false negative, as the test specifically measures the ability to utilize citrate as the sole carbon source, rather than merely the presence of the enzyme. Thus, a negative result could reflect the organism's inability to metabolize citrate in that particular environment.
What is the chemical formula of ammonium citrate?
The chemical formula of ammonium citrate is (NH4)3C6H5O7.
How much water to be used in the preparation of ammonium citrate?
Typically, you would use enough water to dissolve the desired amount of ammonium citrate completely. The ratio of water to ammonium citrate can vary based on the specific recipe or application, but a common guideline is to use approximately 1 part ammonium citrate to 3 parts water by weight.
Can you make ammonium citrate from citric acid and ammonium hydroxide?
Yes, you can make ammonium citrate from citric acid and ammonium hydroxide. Simply mix the two compounds in water, neutralize the citric acid with ammonium hydroxide until it dissolves completely, and then evaporate the solution to obtain solid ammonium citrate.
How do you make Ammonium ferric citrate?
Ammonium ferric citrate can be made by reacting ferric citrate with ammonium hydroxide. First, dissolve ferric citrate in water and then add a solution of ammonia until the desired pH is reached, followed by evaporation to get the final product.
How much elemental iron in 100 mg ferric ammonium citrate?
Ferric ammonium citrate contains about 20% elemental iron. So, in 100 mg of ferric ammonium citrate, there would be approximately 20 mg of elemental iron.
What is the molar mass of ferric ammonium citrate?
The molar mass of ferric ammonium citrate is approximately 270.00 g/mol.
Home come E.coli can't grow on Simmons Citrate Media?
E.coli is unable to grow on Simmons Citrate Media because it lacks the ability to utilize citrate as a carbon source. This is due to the absence of the citrate permease enzyme needed to transport citrate into the cell for metabolism. As a result, E.coli is unable to grow on this type of media.
Ferrous fumarate ferric ammonium citrate iron dextrose?
These are different forms of iron supplements commonly used to treat iron deficiency anemia. Ferrous fumarate and iron dextrose are primarily used orally or through injection, while ferric ammonium citrate can be taken both ways. The choice of supplement depends on factors like the severity of the anemia and individual tolerance.
What happens when you mix citric acid baking soda and vinegar?
A mixture of ammonium acetate and citrate is obtained.
Why is ammonium in soft drinks?
Ammonium ferric citrate is a food additive with E number E381 used as an acidity regulator. It is a green or reddish-brown powder which is very soluble in water.
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
Does irn bru have iron in it?
Yes, albeit a very minuscule amount (less than 0.002%), in the form of ammonium ferric citrate.
