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Temperature

As you increase the temperature of the system in which the enzyme is involved in, the rate of catalysis of the enzyme increases. However, one must make take note that the optimum temperature of the enzyme must not be surpassed because very high temperatures could change the structure of the protein-based enzyme, rendering the enzyme ineffective.

Surface Area of the substrate

If you increase the surface area of the substrate, the enzyme will bind to more sites on the substrate and the rate of the reaction will therefore be faster.

pH

Different enzymes work in different environments. One of the factors that determine the state of the environment in which the enzyme operates is its pH. There are enzymes that exhibit optimum rate of catalysis of reactions at in an acidic environment while there are some that exhibit their optimum rate at a basic environment, while yet others only operate in relatively neutral environments.

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