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Your results might be different then these but I hope this will give you an idea for an answer
a. In our lab we observed growth in all of the test tubes. Originally we had hypothesized that the tubes with higher concentrations of disinfectant would inhibit the growth of the inoculated organism. This experiment allows us to conclude that the disinfectant is not very efficient. Although we were able to come to a conclusion, our results are limited to the organisms that we used to inoculate the test tubes. Different organisms might be more resistant to the disinfectant. A conclusion that the disinfectant we used is inefficient with all bacteria cannot be made.
What else can I help you with?
What is the phenol coefficient?
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
What is the result when a Lucas test is done on a Phenol?
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
Why FeCl2 is not used to identify phenol?
Because it's a test for phenols or enols
What are identification tests of phenol?
You add a specific ionic compound in a solution to determine if phenol or any of its derivatives are present. Ferric chloride (FeCl3) for example, can be used for such tests. Basically, when you mix the solution with the compound, the ions will interact with the phenol molecules or groups, and as a result, the solution will briefly change color. If there is no colour change in the solution, this suggest there is no phenol or phenol-based molecules in your solution.
When using the phenol red carbohydrate broth fermentation test at what pH is the pH indicator yellow?
The pH indicator phenol red turns yellow in the fermentation test when the pH drops below 6.8, indicating acid production from fermentation.
What is the phenol coefficient?
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
What is the use of chick-Martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
What is the use of chick martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Does phenol give a positive biuret test?
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
Suggest a chemical test(s) to distinguish ethanol and phenol. Include the reagents reaction conditions and observations. And the equation?
Phenol solution, or phenol directly from the manufacturer?If the phenol is in the form it comes in from the factory, no chemical test is necessary. Phenol is a white crystalline solid.If it's dissolved in water, the easiest test is pH. Phenol solution is more acidic than ethanol. So...whatever you use for a pH test will do ya.
What is the result when a Lucas test is done on a Phenol?
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
What are the limitations of benzidine test?
The benzidine test is a test for cyanide. The limitations of this test are that benzidine has been linked to bladder and pancreatic cancer.
What products contain phenol and how can they be identified?
Phenol is found in various products such as disinfectants, antiseptics, and certain medications. It can be identified by checking the ingredient list on the product label or by conducting a chemical test for phenol.
Why FeCl2 is not used to identify phenol?
Because it's a test for phenols or enols
Why do ibuprofen and naproxen not give a positive test with 1 percent ironIII chloride?
Iron (III) Chloride reacts with the Phenol group. Ibuprofen and Naproxen do not have a phenol group. Acetaminophen, on the other hand, does have a phenol group with would indicate a positive test (purple color change) with in the presence of Iron (III) Chloride.
What test is an alternative to the contingency coefficient?
Cramers V
