Your results might be different then these but I hope this will give you an idea for an answer
a. In our lab we observed growth in all of the test tubes. Originally we had hypothesized that the tubes with higher concentrations of disinfectant would inhibit the growth of the inoculated organism. This experiment allows us to conclude that the disinfectant is not very efficient. Although we were able to come to a conclusion, our results are limited to the organisms that we used to inoculate the test tubes. Different organisms might be more resistant to the disinfectant. A conclusion that the disinfectant we used is inefficient with all bacteria cannot be made.
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
Because it's a test for phenols or enols
You add a specific ionic compound in a solution to determine if phenol or any of its derivatives are present. Ferric chloride (FeCl3) for example, can be used for such tests. Basically, when you mix the solution with the compound, the ions will interact with the phenol molecules or groups, and as a result, the solution will briefly change color. If there is no colour change in the solution, this suggest there is no phenol or phenol-based molecules in your solution.
The pH indicator phenol red turns yellow in the fermentation test when the pH drops below 6.8, indicating acid production from fermentation.
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
Phenol solution, or phenol directly from the manufacturer?If the phenol is in the form it comes in from the factory, no chemical test is necessary. Phenol is a white crystalline solid.If it's dissolved in water, the easiest test is pH. Phenol solution is more acidic than ethanol. So...whatever you use for a pH test will do ya.
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
The benzidine test is a test for cyanide. The limitations of this test are that benzidine has been linked to bladder and pancreatic cancer.
Phenol is found in various products such as disinfectants, antiseptics, and certain medications. It can be identified by checking the ingredient list on the product label or by conducting a chemical test for phenol.
Because it's a test for phenols or enols
Iron (III) Chloride reacts with the Phenol group. Ibuprofen and Naproxen do not have a phenol group. Acetaminophen, on the other hand, does have a phenol group with would indicate a positive test (purple color change) with in the presence of Iron (III) Chloride.
Cramers V