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Studies indicate (Institute of Food Science, Cornell University, etc) that C. sporogenes is effected more by temp and pH than by CO2 or O2. I generally incubate C. sporogenes (tubes) @ 35(plus or minus 2 degree)degree C with caps closed to decrease the available oxygen.

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Account for the fact that the c sporogenes showed a separation within the medium from the bottom of the test tube?

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