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What are the steps to make Vogel-Johnson agar plates?
Prepare a Vogel-Johnson agar base according to the recipe. Autoclave the agar base and cool it to 45-50°C. Add Vogel-Johnson selective supplement to the agar base. Pour the agar mixture into plates and allow it to solidify before use.
Why must warm agar be cooled down at 45 47 celsius before agar plates are poured?
Why we cool agar before pouring depends on WHY we are pouring it. If we are pouring a gel or standard media for streak culture then we allow some cooling to take place because we don't want to burn our fingers. If we are pouring a pour plate we allow a lot of cooling to take place so we don't kill our microbes through heat steralization. As my teacher used to say "boiled bugs don't grow".
How does the bacteria remain uncontaminated when the covers slightly ajar after pouring nutrient agar into petri dishes?
This may be a better way to ask this... When pouring Nutrient agar into Petri plates, the procedure instructs you to keep the covers slightly ajar. Explain why the plates don't get contaminated from organisms suspended in the air?
What is alger plates?
Do you mean "agar plates"? Agar agar is a seaweed that, when boiled, makes a product similar to gelatin. This stuff is edible, so vegans who liked jello can have it. It's used by boiling it in water, adding things to it, pouring it into petri dishes and letting it harden to use in making bacteria cultures. Some of the things that are added to it are beef broth--"nutrient agar"--and blood--"blood agar."
What might the consequence be of pouring the plates 2mm deep instead of 4mm?
Pouring plates at 2mm depth instead of 4mm could result in a reduced volume of solid agar available for bacterial growth, potentially leading to decreased colony size and difficulty in isolating or visualizing colonies. Additionally, lower volume may lead to faster agar evaporation, potentially drying out the plates more quickly, affecting the overall quality of the experiment.
What are unopened agar plates referred called?
Unopened agar plates are typically referred to as "sterile agar plates."
What are the steps to make Vogel-Johnson agar plates?
Prepare a Vogel-Johnson agar base according to the recipe. Autoclave the agar base and cool it to 45-50°C. Add Vogel-Johnson selective supplement to the agar base. Pour the agar mixture into plates and allow it to solidify before use.
Why does one temper agar?
It allows the agar to cool, if the agar is too hot it could kill the bacteria if you are pouring it right onto a sample, if you are pouring it into plates to use later it just makes it easier to pour as you will not be burning your hand off
Why must warm agar be cooled down at 45 47 celsius before agar plates are poured?
Why we cool agar before pouring depends on WHY we are pouring it. If we are pouring a gel or standard media for streak culture then we allow some cooling to take place because we don't want to burn our fingers. If we are pouring a pour plate we allow a lot of cooling to take place so we don't kill our microbes through heat steralization. As my teacher used to say "boiled bugs don't grow".
How does the bacteria remain uncontaminated when the covers slightly ajar after pouring nutrient agar into petri dishes?
This may be a better way to ask this... When pouring Nutrient agar into Petri plates, the procedure instructs you to keep the covers slightly ajar. Explain why the plates don't get contaminated from organisms suspended in the air?
What is alger plates?
Do you mean "agar plates"? Agar agar is a seaweed that, when boiled, makes a product similar to gelatin. This stuff is edible, so vegans who liked jello can have it. It's used by boiling it in water, adding things to it, pouring it into petri dishes and letting it harden to use in making bacteria cultures. Some of the things that are added to it are beef broth--"nutrient agar"--and blood--"blood agar."
What might the consequence be of pouring the plates 2mm deep instead of 4mm?
Pouring plates at 2mm depth instead of 4mm could result in a reduced volume of solid agar available for bacterial growth, potentially leading to decreased colony size and difficulty in isolating or visualizing colonies. Additionally, lower volume may lead to faster agar evaporation, potentially drying out the plates more quickly, affecting the overall quality of the experiment.
Prewarm agar plates?
To prewarm agar plates, simply place them in a 37°C incubator for about 30 minutes before use. This ensures that the agar solidifies evenly and prevents condensation from forming on the plates when they are inoculated. Always handle prewarmed plates carefully to maintain sterility.
What is the jelly in agar plates?
boobs
Types of agar plates and their function?
Nutrient agar plates: used for general growth of most bacteria. Blood agar plates: used to differentiate bacteria based on their ability to hemolyze red blood cells. MacConkey agar plates: used to differentiate lactose-fermenting bacteria from non-lactose fermenters based on their ability to grow and ferment lactose.
What are the differences between agar plates and petri dishes, and how do these differences impact their use in laboratory experiments?
Agar plates and Petri dishes are both used in laboratory experiments for growing microorganisms. The main difference between them is that agar plates are the medium used to grow the microorganisms, while Petri dishes are the containers that hold the agar plates. This impacts their use in experiments because agar plates provide a solid surface for the microorganisms to grow on, while Petri dishes provide a sterile environment for the agar plates to be placed in. This allows for the controlled growth and observation of microorganisms in a laboratory setting.
What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
