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  1. Prepare a Vogel-Johnson agar base according to the recipe.
  2. Autoclave the agar base and cool it to 45-50°C.
  3. Add Vogel-Johnson selective supplement to the agar base.
  4. Pour the agar mixture into plates and allow it to solidify before use.
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1y ago

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What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?

Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.


What is the purpose of of agar in nutrient agar?

To make it semi-solid


Why must warm agar be cooled down at 45 47 celsius before agar plates are poured?

Why we cool agar before pouring depends on WHY we are pouring it. If we are pouring a gel or standard media for streak culture then we allow some cooling to take place because we don't want to burn our fingers. If we are pouring a pour plate we allow a lot of cooling to take place so we don't kill our microbes through heat steralization. As my teacher used to say "boiled bugs don't grow".


Which group of algae produce agar agar?

Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)


How Can You Make Agar?

Boil ¼ cup of water. Add one teaspoon of gelatin and one teaspoon of sugar. Stir for one minute until everything is dissolved. 2. Cool the solution for ten minutes. 3. Fill a petri dish to a little below the top edge. 4. Close the petri dish by putting on the cover immediately. Let the covered petri dish stand for at least four hours. Don't touch the growth medium or you'll add your own germs! This recipe will fill two, small petri dishes. If you and your class plan to fill more petri dishes, make more germ food by increasing the amount of your ingredients proportionally.

Related Questions

How do you make a sentence with inoculate?

During the microbiology lab, the students were instructed to inoculate the agar plates with the bacterial culture.


What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?

Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.


How is proper placement of agar plate in the incubator?

An agar plate should be placed upside down in the incubator to keep water drops off the agar and prevent the bacteria from drowning. The bacteria are incubatored at 30-40*c for one to three days. the bacteria feed from the nutrients in the agar and reproduce to form colonies of millions of bacteria. Source: NCEA level one science study guide 1.3 biology AS 90188


What is the purpose of of agar in nutrient agar?

To make it semi-solid


What brands contain Agar?

Agar or agar-agar is gelatin substances that can be obtain from algae. In culinary agar refers to a vegetable gelatin that is being used to make jellies, puddings, and custards products.


How do you make pitri dish gel agar?

Agar is generally made by mixing the powdered form with varying ingredients. The powder is based on seaweed extract for nutrient agar, and nutrient agar is generally a base for most other agars - eg. Horse Blood Agar is nutrient agar with horses blood added; Choc agar has defibrinated (cooked) horses blood added.


What are some creative ways to use agar-agar in vegan cooking?

Agar-agar can be used in vegan cooking as a gelatin substitute to make desserts like puddings, jellies, and fruit gels. It can also be used to thicken soups, sauces, and even as a binder in vegan burgers or meatballs. Additionally, agar-agar can be used to create vegan versions of traditional Asian dishes like agar-agar noodles or agar-agar sushi rolls.


Why must warm agar be cooled down at 45 47 celsius before agar plates are poured?

Why we cool agar before pouring depends on WHY we are pouring it. If we are pouring a gel or standard media for streak culture then we allow some cooling to take place because we don't want to burn our fingers. If we are pouring a pour plate we allow a lot of cooling to take place so we don't kill our microbes through heat steralization. As my teacher used to say "boiled bugs don't grow".


Which group of algae produce agar agar?

Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)


What are the functions of thallophyta?

1) it is used by aquatic organisms as food. 2) it is to make some chemicals like agar-agar.


What are some creative ways to use agar agar in vegan cooking and baking?

Agar agar can be used in vegan cooking and baking as a gelatin substitute. It can be used to make vegan jellies, custards, puddings, and even vegan cheese. Agar agar can also be used to thicken sauces and soups, and to create vegan marshmallows and gummy candies.


What do you add to a liquid media to make it solid?

AGAR