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An agar plate should be placed upside down in the incubator to keep water drops off the agar and prevent the bacteria from drowning. The bacteria are incubatored at 30-40*c for one to three days.
the bacteria feed from the nutrients in the agar and reproduce to form colonies of millions of bacteria.
Source: NCEA level one science study guide
1.3 Biology
AS 90188
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Prewarm agar plates?
before bacterial culture, the media containing agar i.e. solid agar should dry in the incubator, that is prewarm agar plate.
What factors would account for an absence of growth on a streak plate?
* Wrong temperature in the incubator * Not enough time in the incubator * No culture was obtained on the inoculating loop * Wrong type of agar for optimum growth (nutrients,etc.)
What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
What is a Mac plate?
MacConkey Agar plate
Why are there areas on the agar jelly where no bacteria are growing?
There are areas on the agar plate where no bacteria grow because that area was missed when the plate was streaked.
Prewarm agar plates?
before bacterial culture, the media containing agar i.e. solid agar should dry in the incubator, that is prewarm agar plate.
What factors would account for an absence of growth on a streak plate?
* Wrong temperature in the incubator * Not enough time in the incubator * No culture was obtained on the inoculating loop * Wrong type of agar for optimum growth (nutrients,etc.)
Where should a label be written on an agar plate?
On the base of the agar plate.
Where should a label written on an agar plate?
On the base of the agar plate.
What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
Why the inoculated agar medium plate must be inverted position in incubator?
Moisture in the air condenses on the lid of the plate and drops on top the agar if the plates are place right way up. The falling water droplets will spread the bacteria and especially ruin streak plates and spead plates where you need clear distict separate colonies.
What is the purpose of agar in agar?
Agar solidifies media, and will remain solid even when placed in an incubator. Few microorganisms are able to metabolize it, so it won't provide an energy source.
What is a Mac plate?
MacConkey Agar plate
What is the name of the streak when you apply specimen to agar plate?
It is called a streak plate, inoculating the agar plate with a zig-zag motion.
Why are there areas on the agar jelly where no bacteria are growing?
There are areas on the agar plate where no bacteria grow because that area was missed when the plate was streaked.
How are Petri dishes sterilized?
To determine that an agar plate is sterlize you can keep it overnight in incubator without streaking and if there is any growth peresent in it then it is contaminated and if not then it is obveiously sterlize
When is a starch agar plate used?
Agar is used to culture bacteria for scientific research.
