* Wrong temperature in the incubator * Not enough time in the incubator * No culture was obtained on the inoculating loop * Wrong type of agar for optimum growth (nutrients,etc.)
certain species of bacteria may not grow in the agar environment,
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
The areas that are streaked by the inoculating loop will have greater growth than the areas that were not streaked. This is because at some point on the streaks the individual cells will have been removed from the loop as the loop glides along the agar surface and will give rise to separate colonies. As the colonies grow they will spread to other areas that are not contaminated in the agar. This is an easy answer and don't know why someone has not put and answer to it. ---------------------------------------------------------------------------- - I guess the area that this question is talking about is the quadrants in which the streak are placed. therefore the area with greatest amount will be the 1 quadrant (1st streak) because it has the most conc amount of microorganism .. then the 4th quadrant(last streak) has the least amount of growth and it's main purpose is to isolate the microrganism :) - anonymous :DD
because the growth of the unknown bacterial colony will create a pattern when it is grown that is characteristic and can help identify the unknown organism
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
A streak plate is a surface of unglazed ceramic, used to find the true color of a mineral specimen by drawing the specimen across it. The color of the resultant powder is referred to as the streak or streak color of a mineral.
1. What is the purpose of inverting inoculated plates during incubation?2. Where should colonies appear in the case of : a. Streak plate b. pour plates3. Indicate the temperature ranges for the following microbial categories.a. psychropiles b. mesophiles c. thermopiles4. What factors could account for an absence of growth on a pour plate?5. What factors could account for an absence of growth on a streak plate?6. What explanations could be given for the failure of obtaining isolated colonies on a streak plate?7. Gelatin and Agar comparison:a. Chemical composition b. temperature required for melting and solidifyc. Possibility of enzymatic attack by bacteria, Yes or No.
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
which sector of your streak plates most shows confluent growth?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
Streak-plate method
what is the streak of sedimentary rocks
No. It can vary from the visible color of the mineral and, since visible color of them ineral is not a reliable indicator of type, it is important to learn to use streak, (along with other factors), to correctly identify a mineral.
there is no name for the color, but there is the "streak" of a mineral, meaning the color of its streak.
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
The areas that are streaked by the inoculating loop will have greater growth than the areas that were not streaked. This is because at some point on the streaks the individual cells will have been removed from the loop as the loop glides along the agar surface and will give rise to separate colonies. As the colonies grow they will spread to other areas that are not contaminated in the agar. This is an easy answer and don't know why someone has not put and answer to it. ---------------------------------------------------------------------------- - I guess the area that this question is talking about is the quadrants in which the streak are placed. therefore the area with greatest amount will be the 1 quadrant (1st streak) because it has the most conc amount of microorganism .. then the 4th quadrant(last streak) has the least amount of growth and it's main purpose is to isolate the microrganism :) - anonymous :DD
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.