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Why is motility medium inoculated only one half the way down the tube?
Motility medium is inoculated only halfway down the tube to observe the migration of motile bacteria from the point of inoculation. This allows for the determination of the motility of the bacteria based on their ability to move away from the point of inoculation. If the bacteria are motile, they will spread throughout the medium, resulting in turbidity along the line of inoculation.
Why is urine specimen is inoculated on cled?
Urine specimens are inoculated on CLED (cysteine lactose electrolyte deficient) agar because this medium supports the growth of most urinary pathogens while inhibiting the growth of normal urinary flora. It allows for the differentiation of different bacterial species based on colony characteristics and lactose fermentation. CLED agar is also able to identify the presence of UTI-causing organisms such as E. coli, Proteus, and Enterococcus.
Microbiology - what is SIM test?
Its a test where a semisolid agar called Sulfide-Indole-Motility medium (or SIM medium) is inoculated with a bacteria to test for hydrogen Sulfide, Indole, and Motility of the organism. The medium is inoculated by a swab and stab type method (rub some bacteria on the surface of the medium and stab a straight hole through the medium using a straight wire with the bacteria on it). Incubate the bacteria for about 24 hours and then begin testing.... If hydrogen sulfide is present, it will react with the sodium thiosulfate in the medium and the indicator, ferric ammonium citrate, to produce ferrous sulfide which falls out of solution as a blackish precipitate. The presence of hydrogen sulfide typically means that the bacteria produces the enzyme cysteine desulfanase which breaks up the cysteine in the medium into, among other components, hydrogen sulfide. The Indole portion of the test is performed by adding Kovac's reagent to the inoculated medium. The Kovac's reagent reacts with the indole(if indole is present) to produce a pinkish-red or redish-purple ring around the top of the test tube. If indole isn't present, there will be no color change. The presence of indole means that the bacteria produces tryptophanase, an enzyme which breaks down tryptophan into smaller components, one of which being indole. The Motility aspect of the test is done by checking the medium for turbidity, or "fuzziness". If the medium has become fairly turbid throughout the medium, then the bacteria is motile. If the medium is clear and the only turbid appearance is in the stab line, then the bacteria is non-motile. Unfortunately, the motility aspect of this test typically gives false negative results. Sometimes the temperature that the bacteria was incubated at wasn't optimum for the species, sometimes the bacteria only have weak motility, sometimes the bacteria's flagella can get damaged which would impair motility, etc... The point is, this test is good if you want to know whether or not the bacteria you're testing produces tryptophanase or cysteine desulfanase. The motility aspect of the test is suspect to question, at least if the test result was negative for motility(a large amount of turbidity in the medium is a definite sign of motility and is hard to refute though).
What is another word for the substance in which a wave travels?
A medium.
Where would The particles of a medium will settle?
The particles of a medium will settle at the bottom of a container due to the force of gravity acting upon them. This settling occurs when the particles are denser than the surrounding medium, such as in a liquid or gas. Over time, they may form a sediment layer at the bottom, while lighter particles may remain suspended or float on the surface. The exact position can also be influenced by factors like turbulence, viscosity, and the size of the particles.
Are reflected waves always inverted?
Reflected waves can be inverted or not depending on the medium through which the wave is traveling. In a denser medium, like a solid, waves are typically inverted upon reflection. In a less dense medium, like air, waves may not be inverted upon reflection.
Why is motility medium inoculated only one half the way down the tube?
Motility medium is inoculated only halfway down the tube to observe the migration of motile bacteria from the point of inoculation. This allows for the determination of the motility of the bacteria based on their ability to move away from the point of inoculation. If the bacteria are motile, they will spread throughout the medium, resulting in turbidity along the line of inoculation.
How is quick screening performed in identifying clones?
For checking the clones quick screening of the colonies helps. the colonies grown the selective medium should be patched on the respective medium and allowed to grow in 37degree incubator. the next day the grown colonies should be inoculated in a liquid specific medium and incubated overnight in a shaker. the very next day the cells are pelleted out and BPB and phenol chloroform of 40microliters,20microliters resp.,to a 1.5microliters culture. quick vortexing should be done followed by spin at 12K for 20mins. later the supernatant is separated and loaded on gel.
A pulse reaches the boundary of a medium in which the speed of the pulse becomes higher. Is the reflection of the pulse the same as for the incident pulse or is it inverted?
same
Is the maximum distance that the particles of a medium move from the rest position is what?
Amplitude.
What do you call the maximum distance that the particles of a medium move from the rest position?
The maximum distance that particles of a medium move from their rest position is called amplitude.
What is the equilibrium point in a wave?
The equilibrium point in a wave is the position where there is no net displacement of the medium from its rest position. It is the point where the medium is at rest, unaffected by the wave passing through it.
The maximum distance that the particles of a medium move from the rest position sometimes called the height of the wave is what?
The maximum distance that particles of a medium move from their rest position is called the amplitude of the wave. This measurement indicates the maximum displacement of particles from their equilibrium position as the wave passes through the medium.
What is the maximum distance the particles of the medium carrying the wave move away from their rest position?
A wave's ___________ is a measure of how far the particles in a medium move away from their normal rest position.
What is the maximum distance the medium can be moved from its rest position?
The maximum distance the medium can be moved from its rest position is called the amplitude. This represents the maximum displacement from equilibrium that a wave can achieve.
What is the resting point of a medium called?
the rest position, or where it stops
The distance between the crest of a wave to the rest position of the medium?
The distance between the crest of a wave and the rest position of the medium is called the amplitude of the wave. It represents the maximum displacement of particles in the medium from their equilibrium position when the wave passes through. The larger the amplitude, the more energy the wave carries.
