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Isotopes are variants of a particular chemical element which differ in neutron number, although all isotopes of a given element have the same number of protons in each atom. Isotope Variation Analysis (detection of adulteration in food products or the geographic origins of products using isoscapes) and Isotopic substitution (to determine the mechanism of a chemical reaction via the kinetic isotope effect) are the most frequently used application for isotopes. Another common application for isotope is isotopic labeling, the use of unusual isotopes as tracers or markers in chemical reactions. Normally, atoms of a given element are indistinguishable from each other. However, by using isotopes of different masses, even different nonradioactive stable isotopes can be distinguished by mass spectrometry or infrared spectroscopy. For example, in 'stable isotope labeling with amino acids in cell culture (SILAC)' stable isotopes are used to quantify proteins. If radioactive isotopes are used, they can be detected by the radiation they emit (this is called radioisotopic labeling).
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What is meant by hot and cold radio-isotope labeling with reference to DNA sequencing?
Hot labeling involves incorporating a radioactive isotope into DNA during sequencing, offering high sensitivity but potential hazards. Cold labeling uses non-radioactive isotopes, like fluorescent dyes, for safer detection. Both methods aid in tracking DNA fragments during sequencing.
When a radioactive tracer is used why is it best to use a radioactive isotope that decays into a stable isotope?
The principle behind the use of radioactive tracers is that an atom in a chemical compound is replaced by another atom, of the same chemical element. The substituting atom, however, is a radioactive isotope. This process is often called radioactive labeling.
How is a radioactive isotope different from a stable isotope?
The radioactive isotope is disintegrated in time and emit radiations.
What is the process in which one isotope changes to another isotope?
The process in which one isotope changes to another isotope is called radioactive decay. During this process, the unstable nucleus of an isotope emits radiation in the form of alpha particles, beta particles, or gamma rays to transform into a more stable isotope. The rate at which radioactive decay occurs is measured by the isotope's half-life.
What is heavy DNA?
"Heavy DNA" refers to DNA labeled with a heavy isotope, such as nitrogen-15 or carbon-14, for research purposes. By incorporating these heavy isotopes, scientists can track the movement and replication of DNA in experiments.
What stable isotopes are used in labeling peptides?
Stable isotopes used in labeling peptides may include 2H, 15N & 13C, respectively, or a combination of 15N & 13C.Source: a passage named Stable Isotope Labeled Peptideson the website of Creative Peptides.
What is meant by hot and cold radio-isotope labeling with reference to DNA sequencing?
Hot labeling involves incorporating a radioactive isotope into DNA during sequencing, offering high sensitivity but potential hazards. Cold labeling uses non-radioactive isotopes, like fluorescent dyes, for safer detection. Both methods aid in tracking DNA fragments during sequencing.
What has the author Robert R Wolfe written?
Robert R. Wolfe has written: 'Isotope tracers in metabolic research' -- subject(s): Metabolism, Methodology, Radioactive tracers in biochemistry, Research 'Radioactive and stable isotope tracers in biomedicine' -- subject(s): Isotope Labeling, Mass Spectrum Analysis, Metabolism, Methodology, Methods, Radioactive tracers in biochemistry, Research
What has the author Erwin Regoeczi written?
Erwin Regoeczi has written: 'Iodine-Labeled Plasma Proteins' -- subject(s): Analysis, Blood proteins, Iodine, Isotope Labeling, Isotopes, Radioiodination
What are the various isotope activities that can be conducted in a laboratory setting?
In a laboratory setting, various isotope activities can be conducted, such as radiometric dating, isotopic labeling, and isotopic analysis. These activities involve using different isotopes to study the age of materials, track chemical reactions, and analyze the composition of substances.
When a radioactive tracer is used why is it best to use a radioactive isotope that decays into a stable isotope?
The principle behind the use of radioactive tracers is that an atom in a chemical compound is replaced by another atom, of the same chemical element. The substituting atom, however, is a radioactive isotope. This process is often called radioactive labeling.
What affects half-life?
The half-life of a radioactive isotope is not affected by environmental conditions, such as temperature and pressure. For all practical purposes, it can be considered as fixed.
What is school labeling?
If you mean as in School labeling (labeling people) Its when someone calls you like an emo or a geek etc ...
What is the radio labeling technique?
Radio labeling is a technique used in biology to track the movement of substances within biological systems, such as cells or organisms. By incorporating a radioactive atom into a molecule of interest, researchers can trace the pathway of the molecule using radiation detection methods. This technique is commonly used in studies involving metabolism, protein synthesis, and other biological processes.
A sentence using and labeling verb?
My teacher was labeling the classroom;
How is a radioactively labeled probe made?
A radioactively labeled probe is made by attaching a radioactive isotope to a nucleic acid molecule, such as DNA or RNA, which is complementary to the target sequence of interest. This is typically done by incorporating the radioactive nucleotide during the synthesis of the probe or by labeling the probe post-synthesis through various chemical methods. The choice of isotope, such as phosphorus-32 or sulfur-35, depends on the specific application and detection requirements. After labeling, the probe can be used in techniques like hybridization to detect specific nucleic acid sequences in various biological samples.
How isotopes can be used to study biological processes?
Isotopes can be injected into a person in order to make MRI / x-ray scans more accurate. When they inject an isotope into you (like say an iodine isotope) they can see exactly where that isotope goes throughout your body (through the blood vessels if injected or through your gastrointestinal tract if swallowed).
