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What is the advantage of API 20E system over the Enterotube II systeme?
API 20E system is advantageous over Enterotube II system for bacterial identification in clinical laboratories because it can provide a more comprehensive identification of Enterobacteriaceae and non-fermenting gram-negative bacteria. It is also known for its user-friendly format and interpretation system, making it easier for technicians to use. Additionally, API 20E system is more standardized and has a higher accuracy rate compared to the Enterotube II system.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
What test is used to further identify unknown gram negative?
The biochemical test commonly used to further identify unknown gram-negative bacteria is the API 20E test kit. This kit contains a panel of biochemical tests that help in identifying members of the Enterobacteriaceae family based on their metabolic traits. The test results are interpreted by comparing them to a database of known bacterial profiles.
How do you identify enterobacter gergoviae?
Enterobacter gergoviae can be identified through various laboratory tests such as biochemical tests, including API 20E or VITEK system. It typically does not ferment lactose and produces a characteristic pink-red pigment on certain media. Additionally, molecular techniques like PCR can be used to confirm the presence of specific genetic markers.
What is American Petroleum Institute scale on hydrometers?
The American Petroleum Institute (API) scale on hydrometers is an arbitrary scale based on Relative Density (Specific Gravity) where - API = (141.5 divided by the Relative Density at 60oF) - 131.5
What is the advantage of API 20E system over the Enterotube II systeme?
API 20E system is advantageous over Enterotube II system for bacterial identification in clinical laboratories because it can provide a more comprehensive identification of Enterobacteriaceae and non-fermenting gram-negative bacteria. It is also known for its user-friendly format and interpretation system, making it easier for technicians to use. Additionally, API 20E system is more standardized and has a higher accuracy rate compared to the Enterotube II system.
What is the purpose of water in the tray of an API 20E system?
The purpose of water in the tray of an API 20E system is to create a humid environment that helps the bacteria's growth and metabolism during the biochemical testing process. This process is essential for accurate identification of the organism based on its biochemical reactions.
What are the results for Api 20 e system 1214373?
The API 20E system is a biochemical test used for the identification of Enterobacteriaceae and other non-fastidious gram-negative bacteria. The results for a specific test, such as the one with the reference number 1214373, would typically include a profile of the organism's metabolic capabilities based on various carbohydrate and enzyme reactions. To obtain specific results for that reference number, you would need to consult the laboratory report associated with that test, as it contains detailed interpretations of the biochemical reactions observed.
Explain advantages and disadvantages of the API 20E system?
The API 20E system offers several advantages, including its ability to rapidly identify a wide range of Enterobacteriaceae and other Gram-negative bacteria through a standardized, easy-to-use format. It requires minimal hands-on time and provides clear results, making it efficient for laboratories. However, disadvantages include its reliance on manual interpretation, which can introduce errors, and the potential for false negatives or positives, especially with atypical strains. Additionally, it may not be suitable for all types of bacteria, limiting its applicability in certain diagnostic contexts.
Why do you need a humid environment for the API 20E test?
The API 20e has dry reagents in the tubes. Adding the sample adds the initial moisture, but it must be kept moist during incubation for the reactions to fully develop. Some samples may take up to 48 hours at 37 degrees C.
What tests could you perform to differentiate between Salmonella altendorf Salmonella brezany Salmonella tinda and Salmonella abortus bovis?
20e API kit
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
In API 20 E why is it important to perform the reagent tests last?
Performing the reagent tests last in the API 20E system helps to prevent cross-reactions between the other test results and thus ensure accurate identification of the microorganism based on its biochemical profile. It also helps to minimize the risk of false-positive or false-negative results that could occur if the reagents interact with the components of the other tests.
Why should the fourth digit in the API 20E profile number be equal to or greater than 4?
The fourth digit must be equal to or greater than four because if the test for GLU is negative, the bacteria is not enterobacteria. This would defeat the purpose of the API 20E test. GLU has a number of four, so even if the GEL or VP test is negative, the fourth digit for that bracket of three tests will be at equal to four.
Why should the first digit in the five digit enteropluri test id value be equal to or greater than 4 and the fourth digit in the API 20E profile number be equal to or great er than 4?
The first digit in the five-digit Enteropluri test ID value being equal to or greater than 4 indicates that the organism is a member of the Enterobacteriaceae family, which is essential for accurate identification. Similarly, the fourth digit in the API 20E profile number being equal to or greater than 4 signifies that the test results correspond to specific biochemical characteristics typical of enteric bacteria. This standardization ensures reliable and consistent interpretation of test results, facilitating proper identification and diagnosis.
Why is it important to check the Gram stained film of the organism before setting up the API 10s strip?
It is important to check the Gram stained film of the organism before setting up the API 10s strip because the API system relies on accurate identification of the organism for determining biochemical reactions. Gram staining helps in determining the type of bacteria (Gram-positive or Gram-negative), which can guide in interpreting API test results accurately. Incorrect identification based on Gram staining can lead to misinterpretation of API results and incorrect identification of the organism.
How does Google SERP API work?
Google SERP API, also known as Google Search API, is a programmatic way to access Google search engine results. Here is how it works: Sign up for a Google Search API provider: To use the Google SERP API, you first need to sign up for an API provider, such as Serphouse. You may need to create an account, set up payment, and obtain an API key. Submit a search query: Once you have signed up for a Google Search API provider, you can use their API to submit a search query. You can specify the search term, search engine location, language, and other search parameters. Retrieve search results: The Google Search API provider will retrieve search results from Google's search engine and return them to you in a structured format, such as JSON or XML. The search results may include search engine snippets, URLs, titles, and other metadata. Parse and analyze the search results: Once you have received the search results, you can use a programming language or a software tool to parse and analyze the data. You can extract information from the search results, such as the search engine ranking, search volume, and other metrics. Display or use the search results: You can display the search results to the user, use them for SEO analysis, or integrate them into your own application or website. It is important to note that there may be limitations on the use of Google SERP API, such as API usage limits and quotas, and changes in Google's search algorithms or policies may affect the API functionality. Therefore, it is important to choose a reputable and reliable API provider and comply with their terms of service.
