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What are short sequences of nucleotides on either side of the gene to be amplified?
These short sequences of nucleotides are called primers. They are designed to match specific regions flanking the target gene and serve as starting points for DNA synthesis by DNA polymerase during PCR amplification. By binding to these primers, DNA polymerase can initiate replication of the target gene sequence.
What enzymes assembles the complementary nucleotides bases during replication?
During DNA replication, the enzyme DNA polymerase assembles complementary nucleotide bases. It adds nucleotides to the growing DNA strand by matching them with their complementary bases on the template strand. Additionally, RNA primase synthesizes a short RNA primer that provides a starting point for DNA polymerase to begin replication.
Which DNA fingerprinting technique examines the length variation of DNA repeat sequences in human DNA?
The DNA fingerprinting technique that examines the length variation of DNA repeat sequences in human DNA is called Short Tandem Repeat (STR) analysis. STRs are short sequences of DNA that are repeated at specific locations in the genome, and the number of repeats can vary among individuals. This variability is used in forensic science, paternity testing, and genetic studies to create unique DNA profiles. By analyzing multiple STR loci, a highly discriminative DNA fingerprint can be generated.
Which assembles the complementary nucleotide bases during replication?
DNA Polymerase
What repeating sequences consists of STR?
Short Tandem Repeats (STRs) are repeating sequences of 2-6 base pairs of DNA that are commonly used in forensic analysis for DNA profiling. These sequences vary between individuals and are highly polymorphic, making them useful for distinguishing one person's DNA from another's. STR analysis involves counting the number of repeats at specific loci to generate a unique genetic profile.
How does shotgun sequencing work to sequence DNA fragments?
Shotgun sequencing breaks DNA into small fragments, sequences them, and then assembles the fragments to create the full DNA sequence. The process involves randomly breaking the DNA into pieces, sequencing each piece, and then using overlapping sequences to piece together the entire DNA sequence.
What are short sequences of nucleotides on either side of the gene to be amplified?
These short sequences of nucleotides are called primers. They are designed to match specific regions flanking the target gene and serve as starting points for DNA synthesis by DNA polymerase during PCR amplification. By binding to these primers, DNA polymerase can initiate replication of the target gene sequence.
What are two essential types of nucleotide sequences found in transposon DNA?
Terminal inverted repeats (TIRs) and target site duplications (TSDs) are two essential types of nucleotide sequences found in transposon DNA. TIRs are short inverted sequences found at each end of the transposon, while TSDs are short repeated sequences created upon insertion of the transposon into the target DNA.
What enzymes assembles the complementary nucleotides bases during replication?
During DNA replication, the enzyme DNA polymerase assembles complementary nucleotide bases. It adds nucleotides to the growing DNA strand by matching them with their complementary bases on the template strand. Additionally, RNA primase synthesizes a short RNA primer that provides a starting point for DNA polymerase to begin replication.
Which DNA fingerprinting technique examines the length variation of DNA repeat sequences in human DNA?
The DNA fingerprinting technique that examines the length variation of DNA repeat sequences in human DNA is called Short Tandem Repeat (STR) analysis. STRs are short sequences of DNA that are repeated at specific locations in the genome, and the number of repeats can vary among individuals. This variability is used in forensic science, paternity testing, and genetic studies to create unique DNA profiles. By analyzing multiple STR loci, a highly discriminative DNA fingerprint can be generated.
What protein assembles the RNA strand on the open DNA strand?
RNApolymerase
DNA coils tightly during divisionand assembles into visible?
Chromosomes.
In eukaryotes DNA wraps around histones and assembles into units?
nucleosomes
Which assembles the complementary nucleotide bases during replication?
DNA Polymerase
What repeating sequences consists of STR?
Short Tandem Repeats (STRs) are repeating sequences of 2-6 base pairs of DNA that are commonly used in forensic analysis for DNA profiling. These sequences vary between individuals and are highly polymorphic, making them useful for distinguishing one person's DNA from another's. STR analysis involves counting the number of repeats at specific loci to generate a unique genetic profile.
What parts of DNA molocules are the basis detected by DNA fingerprinting?
DNA fingerprinting detects unique sequences within the non-coding regions of DNA known as variable number tandem repeats (VNTRs) or short tandem repeats (STRs). These repeated sequences are unique to each individual and provide the basis for differentiating between individuals in DNA profiling.
What do biotechnologists use to cut DNA at specfic sequences?
Biotechnologists use enzymes known as restriction endonucleases, or restriction enzymes, to cut DNA at specific sequences. These enzymes recognize particular short sequences of nucleotides and cleave the DNA at or near these sites. This capability allows for precise manipulation of genetic material, which is essential in cloning, genetic engineering, and various molecular biology applications. Additionally, techniques like CRISPR-Cas9 have emerged, enabling targeted editing of DNA sequences with greater specificity and efficiency.
