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You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating). Or try polyacrilamide. It should solve those bands even better.

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Two small restriction fragments of nearly the same base pair size appear as a single band even when the sample is run to the very end of the gel What could be done to resolve the fragments?

To resolve the two fragments, you can increase the percentage of the gel or run the gel for a longer period of time to improve separation based on size differences. Additionally, you may consider using a different type of gel (e.g., higher resolution gel) or using a different buffer system to enhance resolution.


Why would a scientist use a higher versus a lower percent agarose solution when preparing a gel to separate DNA?

Different percentages have different resolving powers. There is no one agarose percentage that is suitable for all sizes of DNA - you must chose the percentage best for resolving the sizes of DNA you are examining. If your agarose concentration is too dense for the size of your DNA fragments, the DNA will barely migrate through the gel. If the agarose concentration is too dilute for the size of your DNA, it will run straight through the gel without resolving into sharp bands. Generally speaking you use higher percentages if you want to resolve smaller DNA fragments and lower percentages if you want to resolve larger DNA fragments. Small DNA fragments need high percentages or else they'd run straight through the gel without being resolved into bands. Large DNA fragments need low percentages to permit them to migrate into the gel.


Are moon fragments part of a moon?

Moon fragments could be part of the moon as well as external bodies that have hit the lunar surface such as asteroids and meteorites.


Do lunar regolith breccia contains crystalline rock fragments and glassy fragments?

Yes, lunar regolith breccia can contain both crystalline rock fragments and glassy fragments. Crystalline rock fragments are typically from the Moon's crust or mantle, whereas glassy fragments are often formed from impact events that melt and rapidly cool the materials. These fragments provide valuable information about the Moon's history and geological processes.


If a comet broke apart could the comas repel each other when near each other?

Possibly, depending on why the comet split apart to begin with. If the comet's nucleus heats up unevenly. Once broken apart, the gas would be released into space, and the fragments will each continue to move apart. Gravity would act to bring the fragments back together, but the mutual gravity of comet fragments would be VERY small.

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What could be done to resolve two fragments that are nearly the same base-pair length and did not appear on the electrophoresis gel?

You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating).


Two small restriction fragments of nearly the same base pair size appear as a single band even when the sample is run to the very end of the gel What could be done to resolve the fragments?

To resolve the two fragments, you can increase the percentage of the gel or run the gel for a longer period of time to improve separation based on size differences. Additionally, you may consider using a different type of gel (e.g., higher resolution gel) or using a different buffer system to enhance resolution.


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