Any process that humans use to produce a desired product through the use of living things (usually microbial living things). For example the brewing of beer is a bioprocess that uses yeast.
You determine coefficient with the formulae:kla= c(Pg/V)^x * v^ywhere kla is coefficientPg - gassed power inputV - volume of liquid in your vesselc - constv - superficial gas velocity (can be calculated when airflow and geometry are knownx,y are in the ranges (0.4 - 0.95) and (0.2 - 0.75) respectivelyOxygen transfer rateOTR= kla * (Cl in - Cl out)where C is the concentration of oxygen in the liquid coming in and out of the vessel
Biochemical engineering involves applying engineering principles to solve problems related to biological systems and processes, with a focus on the production of biochemicals through fermentation, enzyme technology, and genetic engineering. It includes areas such as biofuel production, pharmaceutical manufacturing, environmental biotechnology, and bioprocess optimization. The field offers opportunities for developing sustainable solutions, improving production processes, and creating new products with applications in various industries.
Bioprocessing is referred to conversion of raw material into final product by means of microbes. Final product is either primary or secondary metabolite. Generally, bioprocessing requires a fermenter in which microbes are given optimum growth conditions so that they can convert raw material into final product through their biochemical-metabolic pathways. Chemical processing is referred to conversion of raw material into final product by means of chemical or synthetic procedures.
adv and disadvantages of continuous cultThe advantages include: Cells can be maintained at a constant physiological state because the specific growth rate and the substrate concentration can be set simply by setting the dilution rate (µ = D)Continuous cultures can take advantage of cell immobilization which allows the maintenance of high concentrations of cells in the reactor at low substrate concentrationsMost downstream processing operations (apart from chromatography) operate in a continuous manner. Continuous bioreactors thus fit in will with overall operation of a bioprocess plantContinuous reactors do not need to be shut down and cleaned as regularly as a batch reactor and thus have a shorter "turn-around" time. This also reduces costs associated with cleaning and filling of the reactor.The above advantages give continuous bioreactors a greater productivity than batch bioreactors and thus continuous reactors can be smaller (and thus cheaper) to construct and operateThere are number of reasons why continuous cultures are not widely used in industry. These include:Continuous cultures are unsuitable for products which are predominantly produced when growth ceases. Such products include many antibiotics, toxins produced during endospore production by Bacillus spp. and Clostridium spp. and monoclonal antibodies.When growth ceases, the specific growth rate (µ) = 0. Since at steady state the specific growth rate = dilution rate, any dilution rate greater than zero will cause the population to be washed out. (If µ < D the cells will be washed out).They are unsuitable for producing various fermented foods and beveridges which depend on a complete batch cycle to produce full flavours. You should remember that unlike a batch culture, a continuous culture maintains the cells at a single physiological state (ie. the specific growth rate is set by the dilution rate :µ = D)Contamination or mutation can have a disasterous effect on the operation of a continuous cultures, especially if the contaminant or mutant is able to maintain a lower concentration of the growth limiting substrate than the desired organism.The US-FDA at present will not approve the liscencing of pharmaceuticals produced in continuous cultures. This is because pharmaceuticals must be able to be linked to a batch identification. This is not as easy with continuous cultures.Large scale continuous cultures are still regarded as risky ventures. Many managers are unwilling to take such risks.
The washout point in a bioprocess occurs when the dilution rate of the growth medium is equal to or greater than the specific growth rate of the microorganisms, resulting in the inability of the microorganisms to grow and be retained in the bioreactor. This leads to a loss of biomass and disrupts the steady-state operation of the bioprocess.
Factors that influence protein yield in a bioprocess include the type of organism used, the growth conditions, the fermentation process, and the purification methods employed.
Associate Professor Dr. Fadzilah Adibah
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Bioprocess
Established in 1990 at Jalan Semarak Campus in capital city of Kuala Lumpur, the Department of Bioprocess Engineering is one of the five departments that make up the Faculty of Chemical and Natural Resources Engineering.
As bioprocess engineers, you need to understand complicated biochemical processes in solution and in living organisms, and thus prospective students will be provided with a repertoire of essential tools that will be needed to analyze, quantify and optimize the processes.
The demand for Bioprocess Engineers in Malaysia is enormous. To realize Vision 2020, the government is currently actively pursuing the establishment of biotechnological-based industries in Malaysia. Malaysian government commitment in promoting biotechnology is reflected in the creation of Bio-Valley, which will provide abundant jobs for Bioprocess Engineers. Moreover, graduates can also work in chemical industries as process engineers.
Matriculation or STPM with minimum of B in Mathematics, Chemistry and Physics /Biology
Now, a bioprocess engineer is defined as a person who employs mathematical-based engineering analysis to explain the physical, biological and chemical processes of complex biological systems and design processes that will yield products that can serve the needs of society such as biopharmaceuticals, food, treated waste-water, DNA recombinant products, agricultural chemicals and many more.
Presently, there are 21 academic staffs and 4 laboratory technicians working on different aspect of the bioprocess engineering.
Bioprocess Engineering Principles Involving the work with bioreactors and heat exchangers. Instrumentation related to biotech. (Industries making these instruments and standardising them)