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Free boundary electrophoresis is a technique used to separate charged particles or molecules in a solution by applying an electric field across a medium. In this method, the separated analytes migrate at different rates due to their differing charges, sizes, and shapes. The boundary between the particles and the solvent is free to move during the separation process allowing for efficient separation.

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1y ago

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What is a boundary between plates that are moving away from each oher?

A boundary where the plates are moving apart is called a divergent boundary. It is also called a constructive boundary because new crust is made there.


What is the name of the boundary where two plates are moving toward one another?

Convergent boundary.


Some viva voce questions for electrophoresis?

1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).


What is is the boundary when two plates that are moving apart?

It is called a divergent plate boundary.


What do we call the plate boundary where plates are moving towards each other?

Convergence Boundary.


What do you call the plate boundary where plates are moving away from each other?

divergent boundary


Explain the hittorf and moving boundary method for determination of transport number?

The Hittorf method involves measuring the change in concentration of each ion at the electrode before and after electrolysis to determine the transport number. The moving boundary method tracks the movement of ions within a solution during electrolysis using techniques like capillary electrophoresis to determine transport numbers. Both methods provide insights into the efficiency of individual ions in carrying charge through the solution.


What do we call the plate boundary where plates are moving away from each other?

divergent boundary


What is the difference between convergent boundary and convergent plate boundary?

Convergent means where plates are moving together. Divergent means where plates are moving apart.


Why the velocity of fluid is zero at stationary boundary?

The velocity at a stationary boundary is zero because the boundary is not moving, thus preventing the fluid from moving past it. This creates a no-slip condition where the fluid particles in direct contact with the boundary stick to it and do not move. This stagnant layer of fluid at the boundary sets the velocity at zero.


What is the boundary between two moving plates called?

h


What a divergent plate boundaries?

A divergent plate boundary is one where the two plates on either side of the boundary are moving apart; 'di-' meaning away. Convergent plates are moving together.