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A maker lane in electrophoresis is a designated lane in a gel where a molecular weight marker or ladder is run alongside the samples. This marker contains a mixture of DNA, RNA, or protein fragments of known sizes, allowing for the estimation of the sizes of the sample bands based on their position relative to the marker. It serves as a reference to help researchers determine the molecular weight of the unknown samples being analyzed.
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What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
Where can one learn more on gel electrophoresis?
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
Who is the maker of cp?
Lane Merrifield (Billybob)
One lane of an electrophoresis gel shows a 100bp ladder. What is the purpose of this ladder?
It is used as a marker for molecular weight.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
When was Electrophoresis - journal - created?
Electrophoresis - journal - was created in 1980.
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
What has the author B J Haywood written?
B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
Where can one learn more on gel electrophoresis?
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
What causes the absence of bands in gel electrophoresis?
The absence of bands in gel electrophoresis can be caused by factors such as improper loading of samples, insufficient DNA concentration, or issues with the gel or electrophoresis equipment.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
