ex plant are nothing but the part of plant that can be used for plant tissue culture processes like for the regeneration of hybrid plants and so on .
All plants produced from the same explant have the same DNA because they are clones. In plant tissue culture, cells are derived from a single parent plant (explant) and retain the genetic makeup of that parent. Therefore, any plantlets or progeny derived from this tissue culture process will also exhibit identical DNA because they originated from the same source plant.
Plant tissue culture laboratories are used for propagation of plants on a large scale, conservation of rare and endangered plant species, production of disease-free plants, genetic transformation of plants, and research on plant growth and development. They play a crucial role in agriculture, horticulture, forestry, and conservation efforts by providing a controlled environment for the manipulation and multiplication of plant tissues.
Plant tissue culture is a technique of producing clones of plant under controlled sterile condition. IN plant tissue culture, an explant is used to produce the whole plant in quick succession of time. Various steps include in the process are: * Prepare a suitable nutrient medium with carbon, nitrogen source along with the growth regulators. * Isolation of the explants (from the original plant) to be used. * Sterilization of the whole equipments like; vessels, media, explants, test tubes. * inoculation * incubation * hardening * Establishment in the field.
plant tissue culture or cell culture:-it is the technique of in vitro, culture in which isolated plant cells, tissues, organs or even entire plant are subjected to grow in nutrient media in glass containers(tubes,conical flask,petri dishes) under aseptic conditions. Aseptic culture of plant may be of the following types as:-(a) ORGAN CULTURE:- These are cultures of isolated plant organs including cultures derived from root tips,stem tips,leaf primordia or immature part of flowers and immature fruits.(b) EMBRYO CULTURE:- These are culture of isolated immature or mature embryos.(c) CALLUS OR TISSUE CULTURE:- These are culture of tissue arising from disorganized proliferation of cells from segment of plant organs.tissue or callus culture are , generally grown on solid medium as amass of cells.(d) SUSPENSION CULTURE:- These are often called cell cultures, as they represent a lower level of organisation than tissue or callus culture. suspension culture are in vitro cultures of isolated cells and very small cell groups remaining dispersed as they grow in excited liquid media.
Asexual Reproduction
medium is essential for explant establishment 1st -micronutrient is the trace of elemnts that are required by all plant cells vitamins -have catalytic function in enzyme system
All plants produced from the same explant have the same DNA because they are clones. In plant tissue culture, cells are derived from a single parent plant (explant) and retain the genetic makeup of that parent. Therefore, any plantlets or progeny derived from this tissue culture process will also exhibit identical DNA because they originated from the same source plant.
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
Plant tissue culture laboratories are used for propagation of plants on a large scale, conservation of rare and endangered plant species, production of disease-free plants, genetic transformation of plants, and research on plant growth and development. They play a crucial role in agriculture, horticulture, forestry, and conservation efforts by providing a controlled environment for the manipulation and multiplication of plant tissues.
The most important part of the plant tissue culture is to maintain the sterile environment and the plant growth in axenic condition, as the seed growth on a very slow rate, any contaminant will cause the seed to unable to growth. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon completely overwhelm the small plant piece that we are trying to clone.
tissue culture of mango se.
by tissue culture method
Lii Jang Liu has written: 'Tropical plant cell and tissue culture' -- subject(s): Plant biotechnology, Plant cell culture, Plant micropropagation, Plant tissue culture
Roberta H. Smith has written: 'Plant tissue culture' -- subject(s): Laboratory manuals, Plant tissue culture 'In Vitro Propagation of Kalanchoe (Avery's Plant Tissue Culture Series)'
Plant tissue culture usually takes some time to grow. Depending on the culture taken, it can take a couple weeks.
The part of the plant that is taken from a desired plant is called explant
Plant tissue culture is a technique of producing clones of plant under controlled sterile condition. IN plant tissue culture, an explant is used to produce the whole plant in quick succession of time. Various steps include in the process are: * Prepare a suitable nutrient medium with carbon, nitrogen source along with the growth regulators. * Isolation of the explants (from the original plant) to be used. * Sterilization of the whole equipments like; vessels, media, explants, test tubes. * inoculation * incubation * hardening * Establishment in the field.