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Retention time is the time of passing material(fluids) through the volume of the drum with determined flowrate :
Retention time=V/Q
which Q : flowrate
and V : Volume
but Detention time is the time that materials stay in drum
in batch systems Detention time equals with Retention time.
but in continious systems or semi batch systems denention time is bigger than Retention timecommonly.
Detention time= n Retention time
which n is the number of times which materials(fluids) pass through the the volume of drum.
What else can I help you with?
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
Are there any advantages in increasing retention time?
The retention time would increase becasue longer distance would be travelled by the analyte!
How will calculate rrf in hplc?
To calculate relative retention factor (RRF) in HPLC, you need to divide the retention time of the compound of interest by the retention time of the reference compound. The formula is RRF = (Retention time of compound of interest) / (Retention time of reference compound). This value helps in comparison and identification of compounds in the chromatogram.
Retention time in Hplc?
Retention time in High Performance Liquid Chromatography (HPLC) refers to the time it takes for a compound to travel through the chromatography column and elute from the detector. It is a key parameter for identifying and characterizing compounds in a sample. Retention time is influenced by factors such as the column type, mobile phase composition, and compound properties.
Can relative retention time changed?
Yes, relative retention time can change based on various factors such as changes in column temperature, flow rate, and mobile phase composition. These changes can affect the interaction between the analyte and stationary phase, thereby altering the relative retention time.
What is the difference between relative retention time and retention time?
Retention time is the time it takes for a compound to travel from the injection point to the detector in chromatography. Relative retention time is the ratio of the retention time of a compound to that of a reference compound in the same chromatographic system. It is used for comparing the behavior of different compounds on the same chromatographic column.
What does retention time mean?
The retention time represents the time it takes to an analyte to pass from the column inlet to the detector.
What is the Retention factor of caffeine?
it depends on the retention time, void time and reagents that you were used on your chromatograph.
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
Which great lake has the shortest retention time?
Lake Erie has the shortest retention time of about 2.7 years
Are there any advantages in increasing retention time?
The retention time would increase becasue longer distance would be travelled by the analyte!
What is difference between retention time and detention time?
Retention time is the time of passing material through the volume of the drum with determined flowrate :Retention time=V/Qwhich Q : flowrateand V : Volumebut Detention time is the time that materials stay in drum
Define retention time?
Retention time refers to the time it takes a solute to travel through the chromatography column. It is assigned to the equivalent solute peak.
What will be the effect of pH on retention time?
Changes in pH can alter the degree of ionization of analytes, affecting their interaction with the stationary phase and mobile phase. This can impact retention time by changing the polarity and charge of the analytes, leading to variations in their retention on the column. Different analytes may respond differently to changes in pH, resulting in shifts in retention times.
How will calculate rrf in hplc?
To calculate relative retention factor (RRF) in HPLC, you need to divide the retention time of the compound of interest by the retention time of the reference compound. The formula is RRF = (Retention time of compound of interest) / (Retention time of reference compound). This value helps in comparison and identification of compounds in the chromatogram.
Retention time in Hplc?
Retention time in High Performance Liquid Chromatography (HPLC) refers to the time it takes for a compound to travel through the chromatography column and elute from the detector. It is a key parameter for identifying and characterizing compounds in a sample. Retention time is influenced by factors such as the column type, mobile phase composition, and compound properties.
What is allowable range of retention time of a peak in hplc analysis if retention time is about 5 minutes?
The allowable range of retention time in HPLC analysis is typically ±0.2-0.3 minutes. Therefore, for a peak with a retention time of 5 minutes, the acceptable range would be around 4.7-5.3 minutes. Any significant deviation outside this range may indicate issues with the analysis.
