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Recently, it has been shown that trehalose and mycolic acids are essential for the growth of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, and important but not indispensable to the survival of Corynebacterium glutamicum. Therefore, to investigate the function of mycolic acids in both the permeability of the cell wall to small nutrients and antibiotics, and the excretion of amino acids by C. glutamicum, a trehalose-deficient mutant of the L-lysine producer ATCC 21527, designated LPtreSotsAtreY, was constructed. By using different carbon sources in either the presence or the absence of external trehalose, a set of endogenously trehalose-free LPtreSotsAtreY cells that exhibited various mycolate contents was generated. The results showed that the structure of the arabinogalactan of these different cell types of LPtreSotsAtreY was not affected when the mycolic acid layer was either missing or impaired. Nevertheless, cells were more susceptible to antibiotics, and the permeability of their cell walls to glycerol was increased. Interestingly, a concomitant increase in the excretion of both L-lysine and L-glutamate was also observed, indicating that the mycolic acid content of the permeability barrier (and not only the peptidoglycan and/or the arabinogalactan) is implicated in the glutamate excretion process.
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http://mic.sgmjournals.org/cgi/content/full/153/5/1424
What else can I help you with?
Does nocardia have mycolic acid?
Yes, Nocardia does have mycolic acid in its cell wall. Mycolic acid is a characteristic component of the cell wall in members of the genus Nocardia, which are classified as acid-fast bacteria due to their resistance to acid-fast staining procedures.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
What drugs specifically targets cell walls that contain arabinogalactan-mycolic acid?
Drugs such as isoniazid and ethambutol specifically target cell walls containing arabinogalactan-mycolic acid in mycobacteria by inhibiting enzymes involved in cell wall synthesis. These drugs are commonly used in the treatment of tuberculosis.
What chemical is found in the cell wall of acid fast organisms that is not found in non acid fast?
The chemical found in the cell wall of acid-fast organisms, such as Mycobacterium species, is mycolic acid. Mycolic acids are long-chain fatty acids that contribute to the waxy, hydrophobic nature of the cell wall, making it resistant to staining and protecting the bacteria from environmental stresses. In contrast, non-acid-fast organisms typically lack this complex lipid structure, resulting in a different cell wall composition. This unique feature is what allows acid-fast bacteria to retain certain stains, such as the Ziehl-Neelsen stain.
What is the significance of the epidermal layer being avascular regarding wound healing?
what is the significance of the epidermal layer being avasculer regardin wound healing
Does nocardia have mycolic acid?
Yes, Nocardia does have mycolic acid in its cell wall. Mycolic acid is a characteristic component of the cell wall in members of the genus Nocardia, which are classified as acid-fast bacteria due to their resistance to acid-fast staining procedures.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
What type of lipid can keep a microorganism from drying out?
Mycolic acid helps these cells survive desiccation and makes them difficult to stain. Organisms with mycolic acid are acid fast.
What makes Mycobacterium resistant to staining?
It has a peptidoglycan layer filled with mycolic acids. The acids make the cell wall waxy and impenetrable to stains. They are classified with gram positive cells because of cell wall thickness and genetic similarities.
Why is E. coli acid fast negative?
Most acid-fast microorganisms belong to the genus Mycobacterium because organisms of this species have a unique property where they have a lipid rich mycolic acid exterior that prevents ordinary dyes from penetrating.When decolorized and counterstained, they maintain the red of the carbolfushin, thus they are acid fast.E. coli is affected by the decolorization by the acid achohol and counterstain by the methylene blue dye because they lack the mycolic acid layer, thus they are non-acid fast.
What is gram positive filamentous forms with mycolic acid in their cell wall?
It is most likely an actinomycete, a type of bacteria known for being filamentous with mycolic acid in their cell wall. Mycolic acid is a characteristic feature of the cell wall of certain bacteria, such as those in the mycobacteria group, including Mycobacterium tuberculosis.
What is a bacterial genus that has waxy mycolic acid in the cell walls?
Mycobacterium is a bacterial genus that has waxy mycolic acid in the cell walls. This acid helps make the cell walls impermeable to many substances, contributing to the resistance of the bacterial cells.
What lipid is responsible for the acid fastness of acid fast organisms?
Mycolic acid is the lipid responsible for the acid-fastness of acid-fast organisms. It is a wax-like lipid found in the cell wall of bacteria such as Mycobacterium and Nocardia, contributing to their resistance to acid-fast staining techniques.
What makes a microorganism non-acid-fast?
The low lipid content in the cells ----------------------------------------------- The above answer is not wrong. I'm just giving adding more information to it. Acid-fast staining is due to the high lipid content (mycolic acid) in the cell wall. Cells that do not have these mycolic acids do not absorb the carbolfuchsin. Microorganisms that have taken up the carbolfuchsin are not easily decolorized by the acid-alcohol step in the preparation procedure.
What is the purpose of the heat during the acid fast staining procedure?
The heat is used to drive the primary stain, carbol fuchsin, into the waxy cell wall of acid-fast bacteria. This allows the stain to penetrate the mycolic acid in the cell wall, making the bacteria resistant to decolorization with acid-alcohol.
What drugs specifically targets cell walls that contain arabinogalactan-mycolic acid?
Drugs such as isoniazid and ethambutol specifically target cell walls containing arabinogalactan-mycolic acid in mycobacteria by inhibiting enzymes involved in cell wall synthesis. These drugs are commonly used in the treatment of tuberculosis.
What makes a microorganism non-acid fast?
The low lipid content in the cells ----------------------------------------------- The above answer is not wrong. I'm just giving adding more information to it. Acid-fast staining is due to the high lipid content (mycolic acid) in the cell wall. Cells that do not have these mycolic acids do not absorb the carbolfuchsin. Microorganisms that have taken up the carbolfuchsin are not easily decolorized by the acid-alcohol step in the preparation procedure.
