Gram +
pH does not directly influence the Gram stain reaction. The Gram stain differentiates between bacterial cell wall structures based on their ability to retain or release crystal violet dye during the staining process. The process is not significantly affected by small changes in pH.
The three types of data obtained from a Gram stain are cell morphology (shape and size of the cells), cell arrangement (how cells are grouped together), and Gram reaction (whether cells are Gram-positive or Gram-negative based on their cell wall composition).
Gram-positive bacteria retain the crystal violet stain and appear purple when stained with Gram's stain, because the thick peptidoglycan layer in their cell wall traps the dye. On the other hand, Gram-negative bacteria do not retain the crystal violet stain and appear red or pink after the addition of a counterstain like safranin, due to their thin peptidoglycan layer which is unable to retain the dye.
Protists are often stained using a silver stain, not a Gram stain.
Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.
Streptococcus pyogenes is gram positive bacteria.
pH does not directly influence the Gram stain reaction. The Gram stain differentiates between bacterial cell wall structures based on their ability to retain or release crystal violet dye during the staining process. The process is not significantly affected by small changes in pH.
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Gram stain
The three types of data obtained from a Gram stain are cell morphology (shape and size of the cells), cell arrangement (how cells are grouped together), and Gram reaction (whether cells are Gram-positive or Gram-negative based on their cell wall composition).
Gram-positive bacteria retain the crystal violet stain and appear purple when stained with Gram's stain, because the thick peptidoglycan layer in their cell wall traps the dye. On the other hand, Gram-negative bacteria do not retain the crystal violet stain and appear red or pink after the addition of a counterstain like safranin, due to their thin peptidoglycan layer which is unable to retain the dye.
Gram Negative
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
Protists are often stained using a silver stain, not a Gram stain.
The Gram stain is used for bacteria and not for viruses.
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.