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Why did the broth go cloudy when he broke the neck of the flask?
The broth likely went cloudy when the neck of the flask was broken due to the introduction of air and the agitation it caused. This disturbance can cause the proteins and other suspended particles in the broth to aggregate and form larger clumps, leading to turbidity. Additionally, the release of dissolved gases and other compounds when the flask is broken can also contribute to the cloudiness.
Which theory did pasteur disprove by using boiled beef broth and a flask with s-shape tubing?
Louis Pasteur disproved the theory of spontaneous generation by showing that microorganisms do not spontaneously appear in boiled broth when the broth is kept sterile in a flask with an S-shaped neck that prevents outside air and microorganisms from entering. This experiment demonstrated that microorganisms come from preexisting microorganisms, not from non-living matter.
Which theory did pasteur disaprove by using boiled beef broth and flask with s- shaped tube?
cells came from nonliving things
What would have happened is Pasteur had tipped on of his flasks so that the broth in the flask came into contact with the curve of the neck?
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
What variables did Pasteur change in his experiment to test the hypothesis of spontaneous generation?
Pasteur changed the shape of the flask to have a curved neck rather than an open one. This allowed air to enter the flask, but prevented dust particles and microorganisms from directly entering. He also boiled the broth in the flask to sterilize it, which killed any existing microorganisms.
How did the design of Pasteur flask help him successfully refute the hypothesis of spontaneous generation?
It showed that microorganisms can only enter the flask as airborne particles.
Why did the broth turn cloudy when Pastuer cracked the neck of his flask?
Bacteria got in, and multiplied.
Which theory did pasteur disprove by using boiled beef broth and a flask with s shape tubing?
spontaneous generation
What is the recommended protocol for preparing bacterial cultures using Luria Bertani broth?
To prepare bacterial cultures using Luria Bertani broth, follow these steps: Measure out the appropriate amount of LB broth and pour it into a sterile flask. Autoclave the LB broth to sterilize it. Inoculate the sterile LB broth with the bacterial culture. Incubate the flask at the appropriate temperature for the bacteria being cultured. Monitor the growth of the bacteria by measuring optical density or performing colony counts. Use the bacterial culture for experiments or storage as needed.
Who in the 1800's performed an experiment using broth in a sealed and unsealed flask to prove spontaneous generation wrong?
Pasteur
Which theory did Pasteur disprove by using boiled beef broth and flask with Shaped tubing?
He disproved that living cells come from nonliving things.
Which theory did pasteur disprove by using boiled beef broth and a flask with s-shape tubing?
Louis Pasteur disproved the theory of spontaneous generation by showing that microorganisms do not spontaneously appear in boiled broth when the broth is kept sterile in a flask with an S-shaped neck that prevents outside air and microorganisms from entering. This experiment demonstrated that microorganisms come from preexisting microorganisms, not from non-living matter.
Which theory did pasteur disaprove by using boiled beef broth and flask with s- shaped tube?
cells came from nonliving things
What would have happened is Pasteur had tipped on of his flasks so that the broth in the flask came into contact with the curve of the neck?
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
How did the design of Pasteur's flask help him successfully refute the hypothesis of spontaneous generation?
With previous attempts to disprove spontaneous generation, they argued that nothing was showing up because when something did, it suffocated. With a swan necked flask, as it's called, air could come in and out of it while the bend in the neck stopped airborne bacteria from infecting the broth.
How did the design of Pasteur's flaskhelp him successfully refute the hypothesis of spontaneous?
The design of Pasteur's flask, with its long, curved neck, allowed air to enter the flask but prevented dust and microorganisms from falling into the sterile broth. By demonstrating that sterile broth remained free of microorganisms unless contaminated by outside sources, Pasteur refuted the hypothesis of spontaneous generation.
What variables did Pasteur change in his experiment to test the hypothesis of spontaneous generation?
Pasteur changed the shape of the flask to have a curved neck rather than an open one. This allowed air to enter the flask, but prevented dust particles and microorganisms from directly entering. He also boiled the broth in the flask to sterilize it, which killed any existing microorganisms.
