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Primer used in DNA synthesis; Promoter used in RNA synthesis.

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12y ago

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What is the best primer?

The best primer for you will depend on your skin type and concerns. A good general option is a hydrating primer for dry skin, a mattifying primer for oily skin, or a blurring primer for minimizing pores and imperfections. It's always best to try samples or get recommendations from a makeup artist to find the best primer for your specific needs.


What is difference between DNA probe and primer?

A DNA probe is a single-stranded DNA sequence used to detect complementary sequences, whereas a primer is a short single-stranded DNA sequence used to initiate DNA synthesis during PCR. Probes are used to identify specific sequences in a sample, while primers are used to amplify a specific target sequence.


In polymerase chain reaction how many kinds of primer are used?

In polymerase chain reaction (PCR), two types of primers are used: a forward primer and a reverse primer. These short DNA sequences are specific to the target DNA region to be amplified and serve as starting points for DNA synthesis by the DNA polymerase enzyme.


When was aluminium wood primer first released?

Aluminum wood primer was first developed in the 1960s as a paint primer specifically formulated for use on wood surfaces.


What is a fusion primer?

Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector

Related Questions

Is there a difference between a muzzleloader 209 primer an a shotgun primer?

A 209 is a 209 regardless of what it is used for.


Difference between rimfire and centerfire ammo?

A centerfire cartridge has the primer in the middle on the bottom side of the shell, where as the rimfire is the whole bottom.


What considerations should be made when designing a primer with restriction sites for a molecular biology experiment?

When designing a primer with restriction sites for a molecular biology experiment, it is important to consider the compatibility of the restriction sites with the target DNA sequence, the efficiency of the restriction enzyme, and the potential for unintended secondary structures or primer-dimer formation. Additionally, the orientation and location of the restriction sites within the primer should be carefully chosen to ensure successful amplification and downstream applications.


How can I add a restriction site to a primer while ensuring it does not interfere with the primer's function?

To add a restriction site to a primer without interfering with its function, you can place the site at the 5' or 3' end of the primer, away from the region that binds to the target DNA. This way, the restriction site will not disrupt the primer's ability to anneal to the target sequence during PCR or other molecular biology techniques.


How can I efficiently design primers with restriction sites for my molecular biology experiments?

To efficiently design primers with restriction sites for molecular biology experiments, use online tools like Primer3 to select appropriate primer sequences and add desired restriction sites. Ensure the restriction sites are compatible with the chosen enzyme and consider factors like primer length, melting temperature, and GC content for optimal primer design.


What is the difference between a forward and reverse primer in PCR amplification?

In PCR amplification, a forward primer is designed to bind to the template DNA strand in the forward direction, while a reverse primer is designed to bind to the template DNA strand in the reverse direction. These primers help initiate the amplification process by marking the specific region of DNA to be copied.


What has the author John W Ritchie written?

John W. Ritchie has written: 'Biology and human affairs' -- subject(s): Biology 'Primer of hygiene' -- subject(s): Hygiene, Juvenile literature, Physiology


What is the difference between centerfire and rimfire rifles?

A center-fire cartridge has a primer on the base or bottom, as the firing pin or hammer strikes the primer-cap it ignites the powder through a hole ( or two holes) inside the cartridge. A rim-fire has a flat metal rim on the base and no primer (such as a .22cal LR.)


What is the difference in gray gripper primer and white gripper primer by glidden?

White primer is for use as a primer for lighter colors and the grey is best used as and undercoat for darker colors(blacks, dark blues, dark green etc..). A darker top color requires more coats with lighter color primer.


What is the difference between call and put options?

From your question it appears that you need some basic education on this topic. You can get a primer on puts and calls at http://www.safe-options-trading-income.com/


Face primer as eyeshadow primer?

I've used the eyeshadow primer, and can't really see much of a difference between it and just putting foundation on the eyelid. So...I don't buy eye primer anymore.


How can I make a primer for PCR?

To make a primer for PCR, you will need to design a short piece of single-stranded DNA that is complementary to the target DNA sequence you want to amplify. This primer will serve as a starting point for the DNA polymerase to begin replicating the target sequence during the PCR process. You can design the primer using bioinformatics tools and order it from a supplier specializing in molecular biology reagents.