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DNA pre-wash buffer is used in molecular Biology protocols, particularly during DNA purification processes, to remove contaminants and impurities from the sample before the actual purification step. It typically contains a salt solution that helps to stabilize the DNA and reduce the presence of proteins, phenols, and other unwanted substances. By washing the DNA, the buffer ensures higher purity and yield of the target DNA, which is crucial for subsequent analysis or experimentation.

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What is DNA polymerase III?

Dna Pol three is a Dna copying bio-machine - similar more in function than in overall shapes and forms {and cofactors} to Dna Pol I and Dna Pol II - each has It's own special Role [ regarding Dna replication ( synthesis of two new Dna intertwined strands ) ] to perform.


Where in the cell is the pre-mRNA produced?

Pre-mRNA is produced in the nucleus of the cell during the process of transcription. In eukaryotic cells, DNA is transcribed into pre-mRNA, which then undergoes processing to become mature mRNA before it is transported to the cytoplasm for translation.


For DNA polymerase to link nucleotides together as new strands of DNA the first nucleotide must be attached to what because DNA polymerase can only add nucleotides to a pre exsisting strand?

The first nucleotide must be attached to a short RNA primer to provide a free 3' hydroxyl group for DNA polymerase to extend from. DNA polymerase starts adding nucleotides to this RNA primer to begin DNA replication.


During DNA replication DNA polymerase lands at origination sites and forms?

DNA polymerase is instrumental in DNA elongation as it catalyzes the addition of deoxyribonucleoside triphosphates to the 3 prime end hydroxyl group of the DNA chain. DNA polymerase binds on the origin of replication, and forms a pre-replication complex with other proteins. The replication complex unwinds DNA during replication


What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?

A primer molecule is required for DNA polymerase to initiate the addition of nucleotides. This primer provides a starting point for DNA polymerase to begin adding nucleotides in the correct sequence. Once the primer is in place, DNA polymerase can add nucleotides complementary to the template strand.

Related Questions

Is it safe to put muddy clothes in the washer or should I "pre-wash" them?

Its preferred to pre-wash or even do this outside with a hose if possible then i would pre-wash and then wash them with like clothes


What is the purpose of the master mix in PCR?

The purpose of the master mix in PCR is to provide all the necessary components for the reaction, such as DNA polymerase, nucleotides, and buffer, in a pre-mixed and optimized form to ensure efficient and accurate amplification of the target DNA.


What is pre-wash lotion?

lotion used pre-washing. :)


Where is DNA from?

New DNA comes from pre-existing DNA by replication.


What is Heterogeneous nuclear DNA?

Heterogeneous nuclear DNA is the DNA present in the nucleus of eukaryotic cells that is not organized into chromatin. It includes DNA sequences that are actively being transcribed and processed, as well as regions of repetitive elements and non-coding DNA. This dynamic pool of DNA plays a crucial role in gene expression regulation and cellular function.


Is it spelled pre-function or prefunction?

prefunction


Who first invented laundry pre-wash stain remover spray?

George Washington


Why do you have to pre-wash the items being dyed?

so its easier for the dye to stay in the fabric


What is the order of how a cell changes DNA messages to protein?

DNA -> transcription -> pre-mRNA -> mRNA processing -> mRNA -> translation -> protein


What is DNA polymerase III?

Dna Pol three is a Dna copying bio-machine - similar more in function than in overall shapes and forms {and cofactors} to Dna Pol I and Dna Pol II - each has It's own special Role [ regarding Dna replication ( synthesis of two new Dna intertwined strands ) ] to perform.


What is the function of the tongatong?

EmButIdO . .. . . . I loVe U prE . . .


Will the Adidas shorts with polyester French terry body shrink in the wash?

No, they have been pre shrunk.