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What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What is the difference between DNA polymerase I II and III?
DNA polymerase I, II, and III are enzymes involved in DNA replication in prokaryotes. DNA polymerase I is responsible for removing RNA primers during DNA replication and filling the gaps with DNA nucleotides. DNA polymerase II is involved in DNA repair pathways, particularly in response to DNA damage. DNA polymerase III is the main enzyme responsible for synthesizing a new DNA strand during replication. It has a high processivity and is the primary enzyme involved in synthesizing the leading and lagging strands of DNA.
What enzyme attaches and adds the matching nucleotides?
DNA Polymerase III adds nucleotides during DNA replication. DNA Polymerase I also adds nucleotides (to a lesser extent). DNA Pol I is responsible for replacing the primers with dNTPs, these sections are then joined to the rest by DNA Ligase.
How does DNA polymerase function in DNA replication?
DNA Polymerase III is responsible for adding new nucleotides to the strand being created. DNA Polymerase I replaces the primers with DNA nucleotides. The fragments are then joined together by ligase, and a new strand has been created.
What are the elements of DNA polymerase?
DNA polymerase III (not DNA polymerase) is an enzyme that works in association with other enzymes during the replication of a DNA molecule. DNA replication begins when the enzyme, known as helicase unwinds a DNA strand. Helicase unwinds a DNA strand, thus, in the process, separating the two DNA templates. The result of the unwinding of the DNA molecule is the formation of a replication bubble. Once a DNA molecule is unwound, it is not stable. The DNA molecule is untwisted, broken and rearranged by an enzyme called topoisomerase in order to create stability at the ends of a replication bubble. In addition, the DNA replication bubble is further stabilized by a group of protein complexes known as single strand binding proteins.Once the DNA molecule is unwound and stabilized, an enzyme called primase assembles an RNA sequence that is complementary to the adjacent DNA template. The purpose of this initial RNA sequence is to provide a point at which DNA polymerase III can start to add nucleotides to the corresponding DNA template. Unlike RNA polymerase, DNA polymerase III requires an RNA sequence, which is known as a primer. DNA polymerase III can attach a nucleotide only to the 3 prime end of an existing nucleotide sequence. Once a primer is assembled by primase, DNA polymerase III begins its work of adding nucleotides to the 3 prime end of the primer.It is important to note that replication proceeds in two directions, since a DNA replication bubble consists of two DNA templates. Since DNA polymerase III proceeds in the three prime to 5 prime direction at one DNA template, it also has to proceed in the 3 prime to 5 prime direction on the other DNA template. Since the template run in opposite directions, the second template will consist of multiple primers and thus short segments of DNA. These short segments of DNA are known as Okazaki fragments. The Okazaki fragments are created by DNA polymerase three since it is only able to proceed in the 3 prime to 5 prime direction.After DNA polymerase III completes its work, DNA polymerase I begins to replace the RNA nucleotides of the primers with DNA nucleotides. Once DNA polymerase I replaces the RNA nucleotides with DNA nucleotides, DNA ligase joins the Okazaki fragments together and the result is a new DNA template.
What is the name of the two enzymes that help in the duplication of DNA?
More than two enzymes are involved. However, the main ones are DNA Polymerase I and DNA Polymerase III. DNA Polymerase III adds new nucleotides and DNA Polymerase I removes primers.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What specific component does DNA polymerase III require in order to function correctly?
DNA polymerase III requires a primer, which is a short piece of RNA or DNA, in order to function correctly.
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
What enzyme elongates DNA?
DNA polymerase is the main enzyme responsible for elongating DNA strands during DNA replication. It catalyzes the addition of nucleotides to the growing strand in a 5' to 3' direction.
What is the difference between DNA polymerase I II and III?
DNA polymerase I, II, and III are enzymes involved in DNA replication in prokaryotes. DNA polymerase I is responsible for removing RNA primers during DNA replication and filling the gaps with DNA nucleotides. DNA polymerase II is involved in DNA repair pathways, particularly in response to DNA damage. DNA polymerase III is the main enzyme responsible for synthesizing a new DNA strand during replication. It has a high processivity and is the primary enzyme involved in synthesizing the leading and lagging strands of DNA.
What is the difference between DNA polymerase III and DNA polymerase I?
pol 1 - exonuclease activity pol 2 - dna repair pol 3 - primary replication enzyme
Which enzyme is used in the synthesis of new copies of DNA?
In humans, many enzymes are involved in DNA replication. Among them are: DNA polymerase I DNA polymerase III Ligase Primase Helicase DNA polymerase I and III perform the bulk of the actual reproduction--their job is to add nucleotides to the growing strands. The others perform specialized functions and are essential to the process.
What are the four enzymes involved in DNA replication and repair?
The four enzymes involved in DNA replication and repair are DNA polymerase, DNA helicase, DNA ligase, and DNA primase. DNA polymerase synthesizes new DNA strands, DNA helicase unwinds the double helix, DNA ligase joins the Okazaki fragments on the lagging strand, and DNA primase synthesizes RNA primers for DNA polymerase to begin replication.
What enzyme attaches and adds the matching nucleotides?
DNA Polymerase III adds nucleotides during DNA replication. DNA Polymerase I also adds nucleotides (to a lesser extent). DNA Pol I is responsible for replacing the primers with dNTPs, these sections are then joined to the rest by DNA Ligase.
What is the enzyme that synthesizes mRna using DNA as a template?
The enzyme responsible for synthesizing mRNA using DNA as a template is called RNA polymerase. It is essential for the process of transcription in which the genetic information from DNA is transcribed into mRNA molecules.
Which is the main enzyme used for the replication of DNA in ecoli?
The main enzyme used for the replication of DNA in E. coli is DNA polymerase III. It is a highly processive enzyme that synthesizes new DNA strands by adding nucleotides in a 5' to 3' direction. DNA polymerase III works alongside other enzymes and proteins in the replisome complex to accurately copy the entire genome during DNA replication.
