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D = wavelength / NA condensor + NA objective

D being minimum distance at which two points can be resolved....... wave length of light used......condensor and objective are the numerical apertures of the condensor lens and objective lens

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What is resolution of objective lens for 1.25 and 0.25 given wavelength of 520nm?

The resolution of an objective lens is given by the formula R = 0.61 * λ / NA, where R is the resolution, λ is the wavelength, and NA is the numerical aperture. For a 1.25 NA lens with a wavelength of 520nm, the resolution would be approximately 266nm. For a 0.25 NA lens with the same wavelength, the resolution would be around 1330nm.


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The smallest resolution for an optical system is determined by the diffraction limit, which is approximately half the wavelength of the light being used. This means that the resolution is limited by the wave nature of light itself. Therefore, for visible light with a wavelength of around 500 nm, the smallest resolution would be around 250 nm.


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What does resolving power of a microscope depend on?

In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.


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What is resolution of objective lens for 1.25 and 0.25 given wavelength of 520nm?

The resolution of an objective lens is given by the formula R = 0.61 * λ / NA, where R is the resolution, λ is the wavelength, and NA is the numerical aperture. For a 1.25 NA lens with a wavelength of 520nm, the resolution would be approximately 266nm. For a 0.25 NA lens with the same wavelength, the resolution would be around 1330nm.


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