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This isn't easy to answer because 'middle layer' is hard to pin point. From the outside front to the back, this is the order, Conjunctiva, cornea, aqueous humor, pupil (with the iris at both sides), lens, vitreous humor, retina, and then the optic nerve.

The uvea is the middle vascular layer of the eye. It contains the iris, ciliary body and chorid.

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