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1.Take 8 to 12 test tubes and label them..
2. Add 5 ml saline in each tube to provide isotonic medium..
3.Add 5 cc blood sample in each tibe
4.Add 1 ml solution containing antibodies
5.Mix and shake gently
5.Took half of first test tube and add to 2nd tube then took half of 2nd tube and add to 3rd tube and son...It is serial dilution..Leave the last tube as such .dnt add antibodies in it..It is Control
6.Keep all test tubes in water bath at temp 37 deg C for 30 min
7.refrigerate them overnight at 4 deg C
8.Check agglutination in the tubes caused by Ag - Ab complex
9. Check the last tube having agglutination..Suppose its dilution is 1:32,then the titer of Ab will b 32..
BY:::dr sakhawat ali
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Hemagglutination-inhibition test description?
It is the measures of the ability of soluble antigen to inhibit the agglutination of antigen-coated red blood cells by antibodies. In this test, a fixed amount of antibodies to the antigen in question is mixed with a fixed amount of red blood cells coated with the antigen (research on passive hemagglutination). Also included in the mixture are different amounts of the sample to be analyzed for the presence of the antigen. If the sample contains the antigen, the soluble antigen will compete with the antigen coated on the red blood cells for binding to the antibodies, thereby inhibiting the agglutination of the red blood cells.
Why 4HA units as standard in hemagglutination inhibition?
4HA (hemagglutinin units) are commonly used as a standard in hemagglutination inhibition assays because they provide a consistent and reproducible measure of viral activity. This standardization allows for the reliable comparison of results across different experiments and laboratories, facilitating the assessment of antibody responses or the effectiveness of vaccines. Using 4HA ensures that the assay remains sensitive enough to detect variations in hemagglutination inhibition, which is crucial for evaluating immune responses to viruses like influenza.
The procedure indicates that the amount of time th test tube was kept in th boiling water bath should be minimized why was this necessary?
The procedure indicates that the amount of time the test tube was kept boiling in the water bath should be minimized. The water bath should never be allowed to boil dry.
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Hemagglutination-inhibition test description?
It is the measures of the ability of soluble antigen to inhibit the agglutination of antigen-coated red blood cells by antibodies. In this test, a fixed amount of antibodies to the antigen in question is mixed with a fixed amount of red blood cells coated with the antigen (research on passive hemagglutination). Also included in the mixture are different amounts of the sample to be analyzed for the presence of the antigen. If the sample contains the antigen, the soluble antigen will compete with the antigen coated on the red blood cells for binding to the antibodies, thereby inhibiting the agglutination of the red blood cells.
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