Benedict's test is primarily used to detect reducing sugars, a type of carbohydrate macromolecule. The rationale behind the test involves the ability of reducing sugars to reduce copper(II) ions to copper(I) oxide in an alkaline solution, resulting in a color change that can be observed visually. A positive result, indicated by a color change from blue to green, yellow, orange, or red, suggests the presence of reducing sugars in the sample. This test is not applicable for non-reducing sugars or other types of macromolecules like proteins and lipids.
Yes, eggs can test positive in the Benedict's test because they contain glucose, which is a reducing sugar that reacts with the reagent in the test to form a colored precipitate.
No, surcose is a disaccharide without a hemiacetal group
Sodium carbonate is added to increase the pH of the solution.
It is normally benedicts solution. Add about 1/4 of the total sugar solution to a test tube and heat it in a hot water bath at around 50 degrees C for 5 min. For example, heat 40mL of sugar water (C12H22O11 aq ) with 10mL of Benedicts soluton (copper and sodium salts). If it is a positive test it will turn yellow.
Benedicts solution
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prosrdure of benidect solution test
Benedict's test is more sensitive than Fehling's test for detecting reducing sugars in a sample. Benedict's reagent has a lower detection threshold and is known to give more accurate results compared to Fehling's reagent.
why should you add 8 drops of urine in the Benedicts test
A negative Benedict's test would indicate that there isn't any presence of reducing sugars in that particular substance.
Yes, eggs can test positive in the Benedict's test because they contain glucose, which is a reducing sugar that reacts with the reagent in the test to form a colored precipitate.
No, surcose is a disaccharide without a hemiacetal group
Sodium carbonate is added to increase the pH of the solution.
It is normally benedicts solution. Add about 1/4 of the total sugar solution to a test tube and heat it in a hot water bath at around 50 degrees C for 5 min. For example, heat 40mL of sugar water (C12H22O11 aq ) with 10mL of Benedicts soluton (copper and sodium salts). If it is a positive test it will turn yellow.
Most chemical supply companies sell it. You can do an internet search for "retail sale of Benedicts solution".
Benedicts solution
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.